LS-C673883

antibody from LSBio

Targeting: ELP3 FLJ10422, KAT9

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C673883

Provider

LSBio

Product name

ELP3 Antibody LS-C673883

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate. All lanes: ELP3 antibody at 3.82 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 63, 61, 50, 53 KDa. Observed band size: 63 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating ELP3 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of ELP3 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ELP3 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating ELP3 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of ELP3 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ELP3 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating ELP3 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of ELP3 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ELP3 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)