Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-27840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ELP3 Monoclonal Antibody (GT11811)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HeLa, HeLa nuclear, 293T, A431, HeLa, HepG2 Predicted Reactivity: Mouse (96%), Zebrafish (92%), Drosophila (88%), Yeast (84%), Xenopus laevis (92%), Chicken (91%), Rhesus Monkey (100%), Bovine (98%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT11811
- Vial size
- 100 µL
- Concentration
- 2.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ELP3 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ELP3 monoclonal antibody (Product # MA5-27840) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ELP3 in HeLa cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ELP3 monoclonal antibody (Product # MA5-27840) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELP3 antibody detects ELP3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody ELP3 Monoclonal Antibody (GT11811) (Product # MA5-27840) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELP3 Monoclonal Antibody (GT11811) detects ELP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELP3 protein stained by ELP3 Monoclonal Antibody (GT11811) (Product # MA5-27840) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ELP3 was performed in 293T whole cell extracts using 5 µg of ELP3 Monoclonal Antibody (GT11811) (Product # MA5-27840). Samples were transferred to a membrane and probed with ELP3 Monoclonal Antibody (GT11811) as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.
