Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 17673-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS4B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSSTSPNLQ KAIDLASKAA QEDKAGNYEE ALQLYQHAVQ YFLHVVKYEA QGDKAKQSIR AKCTEYLDRA EKLKEYLKNK EKKAQKPVKE GQPSPADEKG NDSDGEGESD DPEKKKLQNQ LQGAIVIERP NVKWSDVAGL EGAKEALKEA VILPIKFPHL FTGKRTPWRG ILLFGPPGTG KSYLAKAVAT EANNSTFFSI SSSDLVSKWL GESEKLVKNL FQLARENKPS IIFIDEIDSL CGSRSENESE AARRIKTEFL VQMQGVGVDN DGILVLGATN IPWVLDSAIR RRFEKRIYIP LPEPHARAAM FKLHLGTTQN SLTEADFREL GRKTDGYSGA DISIIVRDAL MQPVRKVQSA THFKKVRGPS RADPNHLVDD LLTPCSPGDP GAIEMTWMDV PGDKLLEPVV SMSDMLRSLS NTKPTVNEHD LLKLKKFTED FGQEG (1-444 aa encoded by BC039574)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 17673-1-AP (VPS4B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 17673-1-AP (VPS4B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-VPS4B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:1000) with HeLa cells lysate 880ug.