PA5-107119

antibody from Invitrogen Antibodies

Targeting: EMC6 MGC2963, RAB5IFL, TMEM93

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-107119

Provider

Invitrogen Antibodies

Product name

EMC6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 79%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The endoplasmic reticulum membrane complex promotes proteostasis of GABA(A) receptors.

Whittsette AL, Wang YJ, Mu TW
iScience 2022 Aug 19;25(8):104754

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Interactions of EMC3 and EMC6 with neurotransmitter-gated ion channels (A) Co-immunoprecipitation (Co-IP) from primary rat cortical neurons demonstrated endogenous interactions between alpha1 subunits of GABA A receptors and EMC3, EMC6, and a number of alpha1-interacting chaperones (BiP and calnexin) and ERAD factors (Grp94 and VCP). Neurons were plated onto 10-cm dishes at a density of one million per dish. At DIV 12, proteins were extracted for Co-IP. IgG was used as a negative control during the immunoprecipitation. n = 3. (B) Co-IP from primary rat cortical neurons demonstrated endogenous interactions between EMC3/EMC6 and a number of ion channels, including N-methyl-D-aspartate receptors (NMDARs, including NR1, NR2A and NR2B subunits) and nicotinic acetylcholine receptors (nAChR alpha7 subunit). n = 3. (C) Co-IP from mouse cortical homogenates, which were prepared from C57BL/6J mice between 8 and 10 weeks of age, demonstrated endogenous interactions between EMC3/EMC6 and selected ion channels. n = 3. (D) Schematic of the primary sequence of EMC3 and EMC6. R31 and R180 in EMC3 and N22 and D27 in EMC6 were reported to influence the biogenesis of EMC-dependent client proteins. (E) Mutation of R31A or R180A in EMC3 significantly reduced the interaction of EMC3 with GABA A alpha1 subunits. The cDNAs of FLAG-tagged EMC3, either in the wild type (WT) form or carrying appropriate mutations of R31A or R180A, were transiently transfected in HEK293T cells stably expressing alpha1be