LS-C357477

antibody from LSBio

Targeting: MSLN CAK1, MPF

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C357477

Provider

LSBio

Product name

MSLN / Mesothelin Antibody (aa306-576) LS-C357477

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Mesothelin antibody Western blot. All lanes: Anti Mesothelin at 0.5 ug/ml. WB: Recombinant Human Mesothelin Protein 0.5ng. Predicted band size: 43 kD. Observed band size: 43 kD.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Mesothelin using anti-Mesothelin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 69KD. The expected band size for Mesothelin is at 69KD.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Mesothelin antibody IHC-paraffin: Human Lung Cancer Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Mesothelin antibody IHC-paraffin: Human Lung Cancer Tissue.