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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 696302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#696302, RRID:AB_2687055
- Product name
- Purified anti-Mesothelin
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A15125D
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates (15µg protein) from PC3 (negative control), HeLa and NIH3T3 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti- Mesothelin antibody (clone A15125D) and competitor’s antibody used at manufactures recommended concentration (upper) or loading control GAPDH (clone Poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A15125D, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody and GAPDH. Lane M is the MW ladder.
- Conjugate
- Unconjugated
