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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MAB1993 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1993, RRID:AB_1679057
- Product name
- MSLN monoclonal antibody, clone MN-1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant MSLN.
- Antibody clone number
- MN-1
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mesothelin expression in human lung cancer.
New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA.
Isolation and characterization of a monoclonal antibody, K1, reactive with ovarian cancers and normal mesothelium.
Ho M, Bera TK, Willingham MC, Onda M, Hassan R, FitzGerald D, Pastan I
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Mar 1;13(5):1571-5
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Mar 1;13(5):1571-5
New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA.
Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman RJ, Pastan I
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Aug 15;11(16):5840-6
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Aug 15;11(16):5840-6
Isolation and characterization of a monoclonal antibody, K1, reactive with ovarian cancers and normal mesothelium.
Chang K, Pastan I, Willingham MC
International journal of cancer. Journal international du cancer 1992 Feb 1;50(3):373-81
International journal of cancer. Journal international du cancer 1992 Feb 1;50(3):373-81
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blotting using MSLN monoclonal antibody, clone MN-1 (Cat # MAB1993) to detect MSLN at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5).Lane 6 contains 50 ng of CDC25-Fc.Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane.Primary antibody was used at 1 ug/mL followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate.Reprinted with permission from Clin. Cancer Res. 11 (16) : 5840-6.
- Validation comment
- Western Blot (Recombinant protein)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human MSLN recombinant protein at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5) with MSLN monoclonal antibodies at 1:1000 dilution. Lane 6 contains 50 ng of human CDC25 recombinant protein.Top panel: Cat # MAB1993.Bottom panel: Cat # MAB1992.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry using MSLN monoclonal antibody, clone MN-1 (Cat # MAB1993) to detect MSLN in PEFF (effective permeability) human tissue sections treated by antigen retrieval methods.MSLN monoclonal antibody, clone MN-1 (Cat # MAB1993) were used to label these sections as follows : C, MAb MB; and D, MAb MN.Reprinted with permission from Clin. Cancer Res. 11 (16) : 5840-6.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
