V3820

antibody from NSJ Bioreagents

Targeting: MSLN CAK1, MPF

Antibody data

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Validation data

Product number: V3820 - Provider product page
Provider: NSJ Bioreagents
Product name: Mesothelin Antibody / MSLN
Antibody type: Monoclonal
Description: This highly specific Mesothelin antibody is suitable for use in Immunohistochemistry/Western blot applications with human, mouse and rat samples.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Antibody clone number: MSLN/2131
Vial size: 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
Concentration: 0.2 mg/ml, 1 mg/ml
Storage: After reconstitution, the Mesothelin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

MSLN protein structure - V3820 shown in red.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human lung mesothelioma with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human endometrial carcinoma with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human tonsil with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE mouse lung with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE rat stomach with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human lung mesothelioma with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human endometrial carcinoma with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human tonsil with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE mouse lung with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Mesothelin antibody (clone MSLN/2131). These results demonstrate the foremost specificity of the MSLN/2131 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Mesothelin antibody (clone MSLN/2131). These results demonstrate the foremost specificity of the MSLN/2131 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.