Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-88224 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88224, RRID:AB_11029696
- Product name
- Rabbit Polyclonal Mesothelin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Mesothelin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice.
In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice.
The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas.
Feng M, Zhang J, Anver M, Hassan R, Ho M
Journal of Cancer 2011 Mar 1;2:123-31
Journal of Cancer 2011 Mar 1;2:123-31
In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice.
Feng M, Zhang J, Anver M, Hassan R, Ho M
Journal of Cancer 2011 Mar 1;2:123-31
Journal of Cancer 2011 Mar 1;2:123-31
The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas.
Bolander A, Agnarsdóttir M, Strömberg S, Ponten F, Hesselius P, Uhlen M, Bergqvist M
Cancer genomics & proteomics 2008 Nov-Dec;5(6):293-300
Cancer genomics & proteomics 2008 Nov-Dec;5(6):293-300
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mesothelin Antibody [NBP1-88224] - Analysis in control (vector only transfected HEK293T lysate) and MSLN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mesothelin Antibody [NBP1-88224] - Analysis in human cell lines HeLa and U-251MG using anti-MSLN antibody. Corresponding MSLN RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining of human lymph node shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Mesothelin Antibody [NBP1-88224] - Staining in human fallopian tube and lymph node tissues using anti-MSLN antibody. Corresponding MSLN RNA-seq data are presented for the same tissues.
