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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-16378 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mesothelin Monoclonal Antibody (SP74)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is mesothelioma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP74
- Vial size
- 500 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A method for Boolean analysis of protein interactions at a molecular level.
Raykova D, Kermpatsou D, Malmqvist T, Harrison PJ, Sander MR, Stiller C, Heldin J, Leino M, Ricardo S, Klemm A, David L, Spjuth O, Vemuri K, Dimberg A, Sundqvist A, Norlin M, Klaesson A, Kampf C, Söderberg O
Nature communications 2022 Aug 13;13(1):4755
Nature communications 2022 Aug 13;13(1):4755
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Mesothelin Monoclonal Antibody (SP74) (Product # MA5-16378) and a ~50 kDa band corresponding to Mesothelin was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1) and NIH:OVCAR-3 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Mesothelin using anti-Mesothelin Monoclonal Antibody (Product # MA5-16378) in Mesothelioma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
