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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- ELISA [1]
- Flow cytometry [1]
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- Product number
- MAB32652 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Mesothelin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Mesothelin in direct ELISAs.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
AAH09272- Isotype
- IgG
- Antibody clone number
- 420411
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Development of Anti-Human Mesothelin-Targeted Chimeric Antigen Receptor Messenger RNA-Transfected Peripheral Blood Lymphocytes for Ovarian Cancer Therapy.
Activated iNKT cells enhance the anti-tumor effect of antigen specific CD8 T cells on mesothelin-expressing salivary gland cancer.
Hung CF, Xu X, Li L, Ma Y, Jin Q, Viley A, Allen C, Natarajan P, Shivakumar R, Peshwa MV, Emens LA
Human gene therapy 2018 May;29(5):614-625
Human gene therapy 2018 May;29(5):614-625
Activated iNKT cells enhance the anti-tumor effect of antigen specific CD8 T cells on mesothelin-expressing salivary gland cancer.
Makita Y, Kunii N, Sakurai D, Ihara F, Motohashi S, Suzuki A, Nakayama T, Okamoto Y
BMC cancer 2018 Dec 17;18(1):1254
BMC cancer 2018 Dec 17;18(1):1254
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Mesothelin ELISA Standard Curve. Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mesothelin Specificity is Shown by Flow Cytometry in Knockout Cell Line Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B). No staining in the Mesothelin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
