14877-1-AP

antibody from Proteintech Group

Targeting: DHODH

Provider product page for 14877-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 14877-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14877-1-AP, RRID:AB_2091723
Product name: DHODH antibody
Antibody type: Polyclonal
Description: KD/KO validated DHODH antibody (Cat. #14877-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DHODH protein structure - 14877-1-AP shown in red.

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Liu X, Chen Z, Yan Y, Zandkarimi F, Nie L, Li Q, Horbath A, Olszewski K, Kondiparthi L, Mao C, Lee H, Zhuang L, Poyurovsky M, Stockwell BR, Chen J, Gan B
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Quercetin Inhibits Neuronal Pyroptosis and Ferroptosis by Modulating Microglial M1/M2 Polarization in Atherosclerosis.

Li H, Cao Z, Liu C, Wang Y, Wang L, Tang Y, Yao P
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Involvement of ferroptosis in eribulin-induced cytotoxicity in ovarian clear cell carcinoma.

Azumi M, Kusama K, Yoshie M, Nakano S, Tsuru A, Kato T, Tamura K
European journal of pharmacology 2024 May 15;971:176544

Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells.

Watanabe T, Yamamoto Y, Kurahashi Y, Kawasoe K, Kidoguchi K, Ureshino H, Kamachi K, Yoshida-Sakai N, Fukuda-Kurahashi Y, Nakamura H, Okada S, Sueoka E, Kimura S
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GAS41 modulates ferroptosis by anchoring NRF2 on chromatin.

Wang Z, Yang X, Chen D, Liu Y, Li Z, Duan S, Zhang Z, Jiang X, Stockwell BR, Gu W
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PM(2.5)-induced iron homeostasis imbalance triggers cardiac hypertrophy through ferroptosis in a selective autophagy crosstalk manner.

Li T, Sun M, Sun Q, Ren X, Xu Q, Sun Z, Duan J
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CGI1746 targets σ(1)R to modulate ferroptosis through mitochondria-associated membranes.

Zhang Z, Zhou H, Gu W, Wei Y, Mou S, Wang Y, Zhang J, Zhong Q
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Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition.

Cui W, Guo M, Liu D, Xiao P, Yang C, Huang H, Liang C, Yang Y, Fu X, Zhang Y, Liu J, Shi S, Cong J, Han Z, Xu Y, Du L, Yin C, Zhang Y, Sun J, Gu W, Chai R, Zhu S, Chu B
Nature cell biology 2024 Jan;26(1):124-137

N-acetylaspartate from fat cells regulates postprandial body temperature.

Felix JB, Saha PK, de Groot E, Tan L, Sharp R, Anaya ES, Li Y, Quang H, Saidi N, Abushamat L, Ballantyne CM, Amos CI, Lorenzi PL, Klein S, Gao X, Hartig SM
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Cell cycle arrest induces lipid droplet formation and confers ferroptosis resistance.

Lee H, Horbath A, Kondiparthi L, Meena JK, Lei G, Dasgupta S, Liu X, Zhuang L, Koppula P, Li M, Mahmud I, Wei B, Lorenzi PL, Keyomarsi K, Poyurovsky MV, Olszewski K, Gan B
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Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition.

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BRCA1-Mediated Dual Regulation of Ferroptosis Exposes a Vulnerability to GPX4 and PARP Co-Inhibition in BRCA1-Deficient Cancers.

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Mitochondrial regulation of GPX4 inhibition-mediated ferroptosis in acute myeloid leukemia.

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A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression.

Hai Y, Fan R, Zhao T, Lin R, Zhuang J, Deng A, Meng S, Hou Z, Wei G
Pharmacological research 2024 Apr;202:107115

POLQ inhibition attenuates the stemness and ferroptosis resistance in gastric cancer cells via downregulation of dihydroorotate dehydrogenase.

Peng Y, Zheng W, Chen Y, Lei X, Yang Z, Yang Y, Liang W, Sun K, Li G, Yu J
Cell death & disease 2024 Apr 4;15(4):248

SIRT6 drives sensitivity to ferroptosis in anaplastic thyroid cancer through NCOA4-dependent autophagy.

Yang Z, Huang R, Wang Y, Guan Q, Li D, Wu Y, Liao T, Wang Y, Xiang J
American journal of cancer research 2023;13(2):464-474

Dihydroorotate dehydrogenase promotes cell proliferation and suppresses cell death in esophageal squamous cell carcinoma and colorectal carcinoma.

Shi ZZ, Jin X, Li WT, Tao H, Song SJ, Fan ZW, Jiang W, Liang JW, Bai J
Translational cancer research 2023 Sep 30;12(9):2294-2307

Targeting DHODH reveals therapeutic opportunities in ATRA-resistant acute promyelocytic leukemia.

Yang T, Shi X, Li S, Zhao Z, Wang J, Yu P, Li H, Wang R, Chen Z
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Blockade of de novo pyrimidine biosynthesis triggers autophagic degradation of oncoprotein FLT3-ITD in acute myeloid leukemia.

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Oncogene 2023 Nov;42(45):3331-3343

Ferroptosis sensitization in glioma: exploring the regulatory mechanism of SOAT1 and its therapeutic implications.

Sun S, Qi G, Chen H, He D, Ma D, Bie Y, Xu L, Feng B, Pang Q, Guo H, Zhang R
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Cholesterol mediated ferroptosis suppression reveals essential roles of Coenzyme Q and squalene.

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De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence.

Yang C, Zhao Y, Wang L, Guo Z, Ma L, Yang R, Wu Y, Li X, Niu J, Chu Q, Fu Y, Li B
Nature cell biology 2023 Jun;25(6):836-847

Reply to: DHODH inhibitors sensitize to ferroptosis by FSP1 inhibition.

Mao C, Liu X, Yan Y, Olszewski K, Gan B
Nature 2023 Jul;619(7968):E19-E23

SLC25A22 as a Key Mitochondrial Transporter Against Ferroptosis by Producing Glutathione and Monounsaturated Fatty Acids.

Liu Y, Wang Y, Lin Z, Kang R, Tang D, Liu J
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Dihydroorotate dehydrogenase regulates ferroptosis in neurons after spinal cord injury via the P53-ALOX15 signaling pathway.

Li D, Lu X, Xu G, Liu S, Gong Z, Lu F, Xia X, Jiang J, Wang H, Zou F, Ma X
CNS neuroscience & therapeutics 2023 Jul;29(7):1923-1939

ML390 inhibits enterovirus 71 replication by targeting de novo pyrimidine biosynthesis pathway.

Yang Q, Wu C, Zhu G, Ren F, Lin B, Huang R, Hu X, Zhao D, Peng K, Wu Y, Wang Q, Huang C, Zhang D
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SGK2 promotes prostate cancer metastasis by inhibiting ferroptosis via upregulating GPX4.

Cheng L, He Q, Liu B, Chen L, Lv F, Li X, Li Y, Liu C, Song Y, Xing Y
Cell death & disease 2023 Jan 31;14(1):74

Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth.

Liu D, Liang CH, Huang B, Zhuang X, Cui W, Yang L, Yang Y, Zhang Y, Fu X, Zhang X, Du L, Gu W, Wang X, Yin C, Chai R, Chu B
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Feb;10(6):e2204006

Depletion of SOD2 enhances nasopharyngeal carcinoma cell radiosensitivity via ferroptosis induction modulated by DHODH inhibition.

Amos A, Jiang N, Zong D, Gu J, Zhou J, Yin L, He X, Xu Y, Wu L
BMC cancer 2023 Feb 3;23(1):117

Neurotoxic A1 astrocytes promote neuronal ferroptosis via CXCL10/CXCR3 axis in epilepsy.

Liang P, Zhang X, Zhang Y, Wu Y, Song Y, Wang X, Chen T, Liu W, Peng B, Yin J, He F, Fan Y, Han S, He X
Free radical biology & medicine 2023 Feb 1;195:329-342

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation.

Zhang W, Dai J, Hou G, Liu H, Zheng S, Wang X, Lin Q, Zhang Y, Lu M, Gong Y, Xiang Z, Yu Y, Hu Y
Molecular cell 2023 Dec 7;83(23):4352-4369.e8

Cloning and Characterization of Yak DHODH Gene and Its Functional Studies in a Bisphenol S-Induced Ferroptosis Model of Fetal Fibroblasts.

Xu H, Li Y, Li Q, Ma Z, Yin S, He H, Xiong Y, Xiong X, Lan D, Li J, Fu W
Animals : an open access journal from MDPI 2023 Dec 13;13(24)

Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function.

Jiang X, Peng J, Xie Y, Xu Y, Liu Q, Cheng C, Yan P, Xu S, Wang Y, Zhang L, Li H, Li Y, Li B, Han J, Yu D
Cell death and differentiation 2023 Aug;30(8):1931-1942

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression.

Qiu S, Zhong X, Meng X, Li S, Qian X, Lu H, Cai J, Zhang Y, Wang M, Ye Z, Zhang H, Gao P
Cell research 2023 Apr;33(4):299-311

Glucose Starvation Inhibits Ferroptosis by Activating the LKB1/AMPK Signaling Pathway and Promotes the High Speed Linear Motility of Dairy Goat Sperm.

Li Y, Zhang G, Wen F, Xian M, Guo S, Zhang X, Feng X, Hu Z, Hu J
Animals : an open access journal from MDPI 2023 Apr 23;13(9)

Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.

Huang S, Wang Y, Xie S, Lai Y, Mo C, Zeng T, Kuang S, Deng G, Zhou C, Chen Y, Huang S, Gao L, Lv Z
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Designer Exosomes for Targeted Delivery of a Novel Therapeutic Cargo to Enhance Sorafenib-Mediated Ferroptosis in Hepatocellular Carcinoma.

Li X, Yu Q, Zhao R, Guo X, Liu C, Zhang K, Zhang W, Liu J, Yu J, Wang S, Hao Q, Li W, Zhang W, Li M, Zhang Y, Zhang C, Gao Y
Frontiers in oncology 2022;12:898156

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Pal S, Kaplan JP, Nguyen H, Stopka SA, Savani MR, Regan MS, Nguyen QD, Jones KL, Moreau LA, Peng J, Dipiazza MG, Perciaccante AJ, Zhu X, Hunsel BR, Liu KX, Alexandrescu S, Drissi R, Filbin MG, McBrayer SK, Agar NYR, Chowdhury D, Haas-Kogan DA
Cancer cell 2022 Sep 12;40(9):957-972.e10

De novo pyrimidine synthesis is a targetable vulnerability in IDH mutant glioma.

Shi DD, Savani MR, Levitt MM, Wang AC, Endress JE, Bird CE, Buehler J, Stopka SA, Regan MS, Lin YF, Puliyappadamba VT, Gao W, Khanal J, Evans L, Lee JH, Guo L, Xiao Y, Xu M, Huang B, Jennings RB, Bonal DM, Martin-Sandoval MS, Dang T, Gattie LC, Cameron AB, Lee S, Asara JM, Kornblum HI, Mak TW, Looper RE, Nguyen QD, Signoretti S, Gradl S, Sutter A, Jeffers M, Janzer A, Lehrman MA, Zacharias LG, Mathews TP, Losman JA, Richardson TE, Cahill DP, DeBerardinis RJ, Ligon KL, Xu L, Ly P, Agar NYR, Abdullah KG, Harris IS, Kaelin WG Jr, McBrayer SK
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De novo pyrimidine synthesis fuels glycolysis and confers chemoresistance in gastric cancer.

He D, Chen M, Chang L, Gu J, Liu F, Gao X, Ruan Y
Cancer letters 2022 Nov 28;549:215837

Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress.

Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B
Nature communications 2022 Nov 17;13(1):7031

Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.

Soflaee MH, Kesavan R, Sahu U, Tasdogan A, Villa E, Djabari Z, Cai F, Tran DH, Vu HS, Ali ES, Rion H, O'Hara BP, Kelekar S, Hallett JH, Martin M, Mathews TP, Gao P, Asara JM, Manning BD, Ben-Sahra I, Hoxhaj G
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Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma Therapy.

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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Jun;9(17):e2105451

A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.

Wu S, Mao C, Kondiparthi L, Poyurovsky MV, Olszewski K, Gan B
Proceedings of the National Academy of Sciences of the United States of America 2022 Jun 28;119(26):e2121987119

Isoliquiritigenin alleviates liver fibrosis through caveolin-1-mediated hepatic stellate cells ferroptosis in zebrafish and mice.

Huang S, Wang Y, Xie S, Lai Y, Mo C, Zeng T, Kuang S, Zhou C, Zeng Z, Chen Y, Huang S, Gao L, Lv Z
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jul;101:154117

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m(6)A-dependent manner.

Sun S, Gao T, Pang B, Su X, Guo C, Zhang R, Pang Q
Cell death & disease 2022 Jan 21;13(1):73

Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma.

Gwynne WD, Suk Y, Custers S, Mikolajewicz N, Chan JK, Zador Z, Chafe SC, Zhai K, Escudero L, Zhang C, Zaslaver O, Chokshi C, Shaikh MV, Bakhshinyan D, Burns I, Chaudhry I, Nachmani O, Mobilio D, Maich WT, Mero P, Brown KR, Quaile AT, Venugopal C, Moffat J, Montenegro-Burke JR, Singh SK
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3,3'-Diindolylmethane Enhances Fluorouracil Sensitivity via Inhibition of Pyrimidine Metabolism in Colorectal Cancer.

Zhang J, Zou S, Zhang Y, Yang Z, Wang W, Meng M, Feng J, Zhang P, Xiao L, Lee MH, Fang L
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A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers.

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DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.

Mao C, Liu X, Zhang Y, Lei G, Yan Y, Lee H, Koppula P, Wu S, Zhuang L, Fang B, Poyurovsky MV, Olszewski K, Gan B
Nature 2021 May;593(7860):586-590

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Mimura K, Sakamaki JI, Morishita H, Kawazu M, Mano H, Mizushima N
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Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.

Halbrook CJ, Pontious C, Kovalenko I, Lapienyte L, Dreyer S, Lee HJ, Thurston G, Zhang Y, Lazarus J, Sajjakulnukit P, Hong HS, Kremer DM, Nelson BS, Kemp S, Zhang L, Chang D, Biankin A, Shi J, Frankel TL, Crawford HC, Morton JP, Pasca di Magliano M, Lyssiotis CA
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Mitochondrial fusion exploits a therapeutic vulnerability of pancreatic cancer.

Yu M, Nguyen ND, Huang Y, Lin D, Fujimoto TN, Molkentine JM, Deorukhkar A, Kang Y, San Lucas FA, Fernandes CJ, Koay EJ, Gupta S, Ying H, Koong AC, Herman JM, Fleming JB, Maitra A, Taniguchi CM
JCI insight 2019 Jul 23;5(16)

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Cao L, Weetall M, Trotta C, Cintron K, Ma J, Kim MJ, Furia B, Romfo C, Graci JD, Li W, Du J, Sheedy J, Hedrick J, Risher N, Yeh S, Qi H, Arasu T, Hwang S, Lennox W, Kong R, Petruska J, Moon YC, Babiak J, Davis TW, Jacobson A, Almstead NG, Branstrom A, Colacino JM, Peltz SW
Molecular cancer therapeutics 2019 Jan;18(1):3-16

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Wang Y, Bai C, Ruan Y, Liu M, Chu Q, Qiu L, Yang C, Li B
Nature communications 2019 Jan 14;10(1):201

Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells.

Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal GJ, Magalhaes Novais S, Olsinova M, Krobova L, An YJ, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim TL, Hozak P, Sardao VA, Koopman WJH, Ricchetti M, Oliveira PJ, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J
Cell metabolism 2019 Feb 5;29(2):399-416.e10

Pharmacological inhibition of dihydroorotate dehydrogenase induces apoptosis and differentiation in acute myeloid leukemia cells.

Wu D, Wang W, Chen W, Lian F, Lang L, Huang Y, Xu Y, Zhang N, Chen Y, Liu M, Nussinov R, Cheng F, Lu W, Huang J
Haematologica 2018 Sep;103(9):1472-1483

Oncogenic KRAS supports pancreatic cancer through regulation of nucleotide synthesis.

Santana-Codina N, Roeth AA, Zhang Y, Yang A, Mashadova O, Asara JM, Wang X, Bronson RT, Lyssiotis CA, Ying H, Kimmelman AC
Nature communications 2018 Nov 23;9(1):4945

The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.

Hoxhaj G, Hughes-Hallett J, Timson RC, Ilagan E, Yuan M, Asara JM, Ben-Sahra I, Manning BD
Cell reports 2017 Oct 31;21(5):1331-1346

PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition.

Mathur D, Stratikopoulos E, Ozturk S, Steinbach N, Pegno S, Schoenfeld S, Yong R, Murty VV, Asara JM, Cantley LC, Parsons R
Cancer discovery 2017 Apr;7(4):380-390

Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.

Ben-Sahra I, Howell JJ, Asara JM, Manning BD
Science (New York, N.Y.) 2013 Mar 15;339(6125):1323-8

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 14877-1-AP(DHODH antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using DHODH antibody 14877-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 14877-1-AP(DHODH antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 14877-1-AP(DHODH antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue