Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14877-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14877-1-AP, RRID:AB_2091723
- Product name
- DHODH antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated DHODH antibody (Cat. #14877-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.
Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy.
Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
Mitochondrial fusion exploits a therapeutic vulnerability of pancreatic cancer.
Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical Properties.
Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.
Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells.
Pharmacological inhibition of dihydroorotate dehydrogenase induces apoptosis and differentiation in acute myeloid leukemia cells.
Oncogenic KRAS supports pancreatic cancer through regulation of nucleotide synthesis.
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.
Mao C, Liu X, Zhang Y, Lei G, Yan Y, Lee H, Koppula P, Wu S, Zhuang L, Fang B, Poyurovsky MV, Olszewski K, Gan B
Nature 2021 May;593(7860):586-590
Nature 2021 May;593(7860):586-590
Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy.
Mimura K, Sakamaki JI, Morishita H, Kawazu M, Mano H, Mizushima N
The Journal of biological chemistry 2021 Jan-Jun;296:100780
The Journal of biological chemistry 2021 Jan-Jun;296:100780
Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
Halbrook CJ, Pontious C, Kovalenko I, Lapienyte L, Dreyer S, Lee HJ, Thurston G, Zhang Y, Lazarus J, Sajjakulnukit P, Hong HS, Kremer DM, Nelson BS, Kemp S, Zhang L, Chang D, Biankin A, Shi J, Frankel TL, Crawford HC, Morton JP, Pasca di Magliano M, Lyssiotis CA
Cell metabolism 2019 Jun 4;29(6):1390-1399.e6
Cell metabolism 2019 Jun 4;29(6):1390-1399.e6
Mitochondrial fusion exploits a therapeutic vulnerability of pancreatic cancer.
Yu M, Nguyen ND, Huang Y, Lin D, Fujimoto TN, Molkentine JM, Deorukhkar A, Kang Y, San Lucas FA, Fernandes CJ, Koay EJ, Gupta S, Ying H, Koong AC, Herman JM, Fleming JB, Maitra A, Taniguchi CM
JCI insight 2019 Jul 23;5
JCI insight 2019 Jul 23;5
Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical Properties.
Cao L, Weetall M, Trotta C, Cintron K, Ma J, Kim MJ, Furia B, Romfo C, Graci JD, Li W, Du J, Sheedy J, Hedrick J, Risher N, Yeh S, Qi H, Arasu T, Hwang S, Lennox W, Kong R, Petruska J, Moon YC, Babiak J, Davis TW, Jacobson A, Almstead NG, Branstrom A, Colacino JM, Peltz SW
Molecular cancer therapeutics 2019 Jan;18(1):3-16
Molecular cancer therapeutics 2019 Jan;18(1):3-16
Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.
Wang Y, Bai C, Ruan Y, Liu M, Chu Q, Qiu L, Yang C, Li B
Nature communications 2019 Jan 14;10(1):201
Nature communications 2019 Jan 14;10(1):201
Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells.
Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal GJ, Magalhaes Novais S, Olsinova M, Krobova L, An YJ, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim TL, Hozak P, Sardao VA, Koopman WJH, Ricchetti M, Oliveira PJ, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J
Cell metabolism 2019 Feb 5;29(2):399-416.e10
Cell metabolism 2019 Feb 5;29(2):399-416.e10
Pharmacological inhibition of dihydroorotate dehydrogenase induces apoptosis and differentiation in acute myeloid leukemia cells.
Wu D, Wang W, Chen W, Lian F, Lang L, Huang Y, Xu Y, Zhang N, Chen Y, Liu M, Nussinov R, Cheng F, Lu W, Huang J
Haematologica 2018 Sep;103(9):1472-1483
Haematologica 2018 Sep;103(9):1472-1483
Oncogenic KRAS supports pancreatic cancer through regulation of nucleotide synthesis.
Santana-Codina N, Roeth AA, Zhang Y, Yang A, Mashadova O, Asara JM, Wang X, Bronson RT, Lyssiotis CA, Ying H, Kimmelman AC
Nature communications 2018 Nov 23;9(1):4945
Nature communications 2018 Nov 23;9(1):4945
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
Hoxhaj G, Hughes-Hallett J, Timson RC, Ilagan E, Yuan M, Asara JM, Ben-Sahra I, Manning BD
Cell reports 2017 Oct 31;21(5):1331-1346
Cell reports 2017 Oct 31;21(5):1331-1346
Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.
Ben-Sahra I, Howell JJ, Asara JM, Manning BD
Science (New York, N.Y.) 2013 Mar 15;339(6125):1323-8
Science (New York, N.Y.) 2013 Mar 15;339(6125):1323-8
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14877-1-AP(DHODH antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using DHODH antibody 14877-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 14877-1-AP(DHODH antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 14877-1-AP(DHODH antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue