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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-66195 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fbxo16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TPLNHQLLNDR VFEERRALLG KWFDKWTDSQ RRRILTGLLE RCSLSQQKFC CRKLQEKIPA EALDFTTKLP RV
- Concentration
- 0.05 mg/mL
Submitted references FBXO16-mediated hnRNPL ubiquitination and degradation plays a tumor suppressor role in ovarian cancer.
Ji M, Zhao Z, Li Y, Xu P, Shi J, Li Z, Wang K, Huang X, Ji J, Liu W, Liu B
Cell death & disease 2021 Jul 31;12(8):758
Cell death & disease 2021 Jul 31;12(8):758
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Fbxo16 in human cell line SK-MEL-30 shows localization to nucleoplasm, plasma membrane, cytosol and vesicles. Samples were probed using a Fbxo16 Polyclonal Antibody (Product # PA5-66195).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 FBXO16 interacts with hnRNPL. A The interaction protein network of FBXO16 revealed by the BioGRID database ( https://thebiogrid.org/ ). B The interaction proteins of 18 F-box proteins were collected by the BioGRID database. Red represents positive hit. Blue represents negative hit. C The cell lysates from HEK293T cells stable expressing empty vector (EV) or FLAG-FBXO16 were subjected to immunoprecipitation (IP) with FLAG M2 beads, followed by immunoblotting with indicated antibodies. D A2078 cells treated with 10 uM MG132 for 4 h were lysed and cell lysate was subjected to IP assay with anti-hnRNPL antibody, followed by immunoblotting with anti-FBXO16 antibodies. E IP assay was performed to detect the interaction between the endogenous FBXO16 and hnRNPL in OVCAR8 cells treated with MG132. F Schematic diagram of the different FLAG-tagged FBXO16 deletion mutants. G Cell lysates of HEK293T cells transiently expressing either full-length FLAG-FBXO16 or FLAG-FBXO16 mutants were immunoprecipitated with FLAG M2 beads, and immune complexes were blotted with anti-hnRNPL antibody. 293T cells transfected HA-hnRNPL plasmid were lysed and incubated with GST alone or GST-FBXO16 immobilized on GST-Sepharose beads. The bound proteins were then detected by immunoblotting with indicated antibodies. H Schematic diagram of the different FLAG-tagged hnRNPL deletion mutants. I Cell lysates of HEK293T cells transiently expressing either full-length FLAG-hnRNPL (1-583) or FLAG-hnRNPL mutants
