LS-C408125

antibody from LSBio

Targeting: ZP2 ZPA

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C408125

Provider

LSBio

Product name

ZP2 Antibody (aa511-544) LS-C408125

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of ZP2 expression in HELA whole cell lysates (lane 1) and HEPG2 whole cell lysates (lane 2). ZP2 at 82 kD was detected using rabbit anti- ZP2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ZP2 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- ZP2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of ZP2 using anti-ZP2 antibody. ZP2 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ZP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.