CABT-RM127

antibody from Creative Diagnostics

Targeting: ZP2 ZPA

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

CABT-RM127

Provider

Creative Diagnostics

Product name

Rat Anti-Zp2 monoclonal antibody, clone JF-4

Antibody type

Monoclonal

Description

Zona pellucida sperm-binding protein 2 is encoded by the Zp2 gene in murine species. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The mammalian zona pellucida mediates species-specific sperm binding, induction of the acrosome reaction, and prevents post-fertilization polyspermy. ZP2 is expressed during the 2-week growth phase of oogenesis, prior to ovulation and may act as a secondary sperm receptor and is proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL and is exocytosed from cortical granules after fertilization, yielding an N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage plays an important role in the post-fertilization block to polyspermy. Female mice lacking Zp2 gene do not form a stable zona matrix and are sterile.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

JF-4

Vial size

25 _g

Storage

Stable for 1 year at 2-8°C from date of receipt.