- GTX111010 - GeneTex FIS1 antibody

Submitted references The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle.

Moore TM, Zhou Z, Cohn W, Norheim F, Lin AJ, Kalajian N, Strumwasser AR, Cory K, Whitney K, Ho T, Ho T, Lee JL, Rucker DH, Shirihai O, van der Bliek AM, Whitelegge JP, Seldin MM, Lusis AJ, Lee S, Drevon CA, Mahata SK, Turcotte LP, Hevener AL
Molecular metabolism 2019 Mar;21:51-67

Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress.

Bi J, Zhang J, Ren Y, Du Z, Li Q, Wang Y, Wei S, Yang L, Zhang J, Liu C, Lv Y, Wu R
Redox biology 2019 Jan;20:296-306

Melatonin prevents Drp1-mediated mitochondrial fission in diabetic hearts through SIRT1-PGC1α pathway.

Ding M, Feng N, Tang D, Feng J, Li Z, Jia M, Liu Z, Gu X, Wang Y, Fu F, Pei J
Journal of pineal research 2018 Sep;65(2):e12491

Mitochondrial Dynamics in Rat Heart Induced by 5-Fluorouracil.

Zhang D, Ma J
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 21;24:6666-6672

Estrogen receptor α protects pancreatic β-cells from apoptosis by preserving mitochondrial function and suppressing endoplasmic reticulum stress.

Zhou Z, Ribas V, Rajbhandari P, Drew BG, Moore TM, Fluitt AH, Reddish BR, Whitney KA, Georgia S, Vergnes L, Reue K, Liesa M, Shirihai O, van der Bliek AM, Chi NW, Mahata SK, Tiano JP, Hewitt SC, Tontonoz P, Korach KS, Mauvais-Jarvis F, Hevener AL
The Journal of biological chemistry 2018 Mar 30;293(13):4735-4751

Empagliflozin normalizes the size and number of mitochondria and prevents reduction in mitochondrial size after myocardial infarction in diabetic hearts.

Mizuno M, Kuno A, Yano T, Miki T, Oshima H, Sato T, Nakata K, Kimura Y, Tanno M, Miura T
Physiological reports 2018 Jun;6(12):e13741

Laminar shear stress promotes mitochondrial homeostasis in endothelial cells.

Wu LH, Chang HC, Ting PC, Wang DL
Journal of cellular physiology 2018 Jun;233(6):5058-5069

Enhancing fatty acid utilization ameliorates mitochondrial fragmentation and cardiac dysfunction via rebalancing optic atrophy 1 processing in the failing heart.

Guo Y, Wang Z, Qin X, Xu J, Hou Z, Yang H, Mao X, Xing W, Li X, Zhang X, Gao F
Cardiovascular research 2018 Jun 1;114(7):979-991

Ding M, Ning J, Feng N, Li Z, Liu Z, Wang Y, Wang Y, Li X, Huo C, Jia X, Xu R, Fu F, Wang X, Pei J
Journal of pineal research 2018 Jan;64(1)

Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease - UMOD (ADTKD-UMOD).

Kemter E, Fröhlich T, Arnold GJ, Wolf E, Wanke R
Scientific reports 2017 Feb 21;7:42970

Metabolic Stress-Induced Phosphorylation of KAP1 Ser473 Blocks Mitochondrial Fusion in Breast Cancer Cells.

Cheng CT, Kuo CY, Ouyang C, Li CF, Chung Y, Chan DC, Kung HJ, Ann DK
Cancer research 2016 Sep 1;76(17):5006-18

Drp1, Mff, Fis1, and MiD51 are coordinated to mediate mitochondrial fission during UV irradiation-induced apoptosis.

Zhang Z, Liu L, Wu S, Xing D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Jan;30(1):466-76

Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins.

Yu CY, Liang JJ, Li JK, Lee YL, Chang BL, Su CI, Huang WJ, Lai MM, Lin YL
PLoS pathogens 2015 Dec;11(12):e1005350

Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization.

Chao LC, Wroblewski K, Ilkayeva OR, Stevens RD, Bain J, Meyer GA, Schenk S, Martinez L, Vergnes L, Narkar VA, Drew BG, Hong C, Boyadjian R, Hevener AL, Evans RM, Reue K, Spencer MJ, Newgard CB, Tontonoz P
Journal of lipid research 2012 Dec;53(12):2610-9

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 40065

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: Raji 15% SDS PAGE GTX111010 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: mouse brain 15% SDS PAGE GTX111010 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: FIS1 antibody detects FIS1 protein by western blot analysis.A. 50 ?g rat brain lysate/extract 15% SDS-PAGEFIS1 antibody (GTX111010) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000.Red: MitoTrackerR Red CMXRos, a mitochondria tracker.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TTC11 antibody (GTX111010) dilution: 1:500.

GTX111010

Antibody data