Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX630992 - Provider product page
- Provider
- GeneTex
- Product name
- FIS1 antibody [GT12112]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT12112] (GTX630992) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FIS1 antibody [GT12112] detects FIS1 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 15 % SDS-PAGE, and blotted with FIS1 antibody [GT12112] (GTX630992) diluted by 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT12112] (GTX630992) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FIS1 antibody [GT12112] detects FIS1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: FIS1 protein stained by FIS1 antibody [GT12112] (GTX630992) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.