Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-43628 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TTC11 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TTC11 Antibody (9810) [NBP2-43628] - Analysis of whole cell extracts (30 ug) was separated by 15 % SDS-PAGE, and blotted with FIS1 antibody [9810] diluted by 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TTC11 Antibody (9810) [NBP2-43628] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [9810].
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TTC11 Antibody (9810) [NBP2-43628] - An intracellular stain was performed on HepG cells with Glut1 Antibody NB110-39113F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
