LS-C498878

antibody from LSBio

Targeting: FIS1 CGI-135, H_NH0132A01.6, TTC11

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C498878

Provider

LSBio

Product name

FIS1 Antibody LS-C498878

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: store at -20°C. Long term: store at -80°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating FIS1 in MCF-7 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of FIS1 Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: FIS1 Antibody(4ug)+ MCF-7 whole cell lysate(500ug);Lane 3: MCF-7 whole cell lysate (20ug);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating FIS1 in MCF-7 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of FIS1 Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: FIS1 Antibody(4ug)+ MCF-7 whole cell lysate(500ug);Lane 3: MCF-7 whole cell lysate (20ug);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating FIS1 in MCF-7 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of FIS1 Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: FIS1 Antibody(4ug)+ MCF-7 whole cell lysate(500ug);Lane 3: MCF-7 whole cell lysate (20ug);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using FIS1 Antibody at dilution of 1:100

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using FIS1 Antibody at dilution of 1:100

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using FIS1 Antibody at dilution of 1:100