A01932-2

antibody from Boster Biological Technology

Targeting: FIS1 CGI-135, H_NH0132A01.6, TTC11

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01932-2

Provider

Boster Biological Technology

Product name

Anti-TTC11/FIS1 Antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for TTC11/FIS1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study.

Zheng H, Liang G, Guan C, Liu L, Dong J, Zhao J, Tang M, Kong L
Nanomaterials (Basel, Switzerland) 2024 Apr 17;14(8)

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Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control.

Xu Y, Chen B, Yi J, Tian F, Liu Y, Ouyang Y, Yuan C, Liu B
Frontiers in pharmacology 2023;14:1137609

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Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway.

Zhang Q, Hu S, He Y, Song Z, Shen Y, Zhao Z, Zhang Q, Qin L, Zhang Q
Nutrients 2022 Sep 24;14(19)

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A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer's Disease.

Wang J, Liu WJ, Shi HZ, Zhai HR, Qian JJ, Zhang WN
Cells 2022 Sep 13;11(18)

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Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model.

Ni W, Zhou J, Ling Y, Lu X, Niu D, Zeng Y, Qiu Y, Si Y, Wang J, Zhang W, Wang Z, Hu J
Brain research 2022 Sep 1;1790:147978

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of FIS1 using anti-FIS1 antibody (A01932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: human HEK293 whole cell lysates, Lane 10: human A549 whole cell lysates, Lane 11: human Hela whole cell lysates, Lane 12: human Raji whole cell lysates, Lane 13: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.

Additional image