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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- SP7366 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SP7366, RRID:AB_981359
- Product name
- anti FIS1 / TTC11
- Antibody type
- Polyclonal
- Antigen
- Amino acids 11-25 of Human Fis1 protein
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Vial size
- 0.2 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of FIS1 in A) HeLa and B) NIH 3T3 cell lysate using SP7366 FIS1 antibody at 1/:1000 dilution.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Specificity validation of the Fis1 antibody. Fis1 was analyzed in M17 cells stably transfected with vector only, Fis1 RNAi, Fis1 (Fis1wt OE) or left untransfected (negative control). The western blot data showed that M17 cells expressed Fis1 (Vector, Untransfected control) which could be knocked down by Fis1 RNAi. Cells transfected with Fis1 overexpressed Fis1. M17 is a human neuroblastoma cell line.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry of Methcarn-Fixed, Paraffin-Embedded Hippocampus from Control (normal) and Alzheimer brain. Quantification in five AD brains and five age-matched controls indicated that Fis1 was detected in only the soma in ~80% of pyramidal neurons in AD Hippocampus, significantly different from what was seen for Control hippocampal neurons. Source: Wang et al, 2009.
