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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-31305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RC74 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (99%), Zebrafish (84%), Xenopus laevis (87%), Chicken (92%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot using RC74 Polyclonal Antibody (Product # PA5-31305). Sample (30 µg of whole cell lysate). Lane A: IMR32 . 7.5% SDS PAGE. RC74 Polyclonal Antibody (Product # PA5-31305) diluted at 1:2,000.
