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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB6954 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EGLN3/PHD3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human EGLN3/PHD3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) EGLN1/PHD2 or rhEGLN2/PHD1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9H6Z9- Isotype
- IgG
- Antibody clone number
- 700210
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- EGLN3/PHD3 in A549 Human Cell Line. EGLN3/PHD3 was detected in immersion fixed A549 human lung carcinoma cell line, untreated (lower panel) or treated (upper panel) with CoCl2, using Mouse Anti-Human EGLN3/PHD3 Monoclonal Antibody (Catalog # MAB6954) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
