Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-79815 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79815, RRID:AB_1110069
- Product name
- Rabbit Polyclonal RanBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RanBP1 (NB100-79815). Detection: DAB
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RanBP1 Antibody [NB100-79815] - Detection of Human RanBP1 by Immunoprecipitation followed by Western Blot. Samples: Whole cell lysate (0.5 mg) from HeLa cells. Antibodies: Affinity purified rabbit anti- RanBP1 antibody BL1968 (Cat. No. NB100-79815) used at 5 mcg/0.5 mg lysate for IP. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody BL1966 (Cat. No. NB100-79814) at 5 mcg/0.5 mg lysate. Immunoprecipitated RanBP1 was blotted using NB100-79814 at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes (A and B). 25043 West
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RanBP1 antibody NB100-79815 (lot 2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RanBP1 was also immunoprecipitated by the previous lot (lot 1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RanBP1 Antibody [NB100-79815] - Sample: FFPE section of human lung carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- RanBP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB