17460-1-AP

antibody from Proteintech Group

Targeting: IFT140 gs114, KIAA0590, WDTC2

Provider product page for 17460-1-AP

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ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [71]
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Validations
Western blot [1]

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Product number: 17460-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17460-1-AP, RRID:AB_2295648
Product name: IFT140 antibody
Antibody type: Polyclonal
Description: KD/KO validated IFT140 antibody (Cat. #17460-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IFT140 protein structure - 17460-1-AP shown in red.

Submitted references XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis.

Hong R, Tan Y, Tian X, Huang Z, Wang J, Ni H, Yang J, Bu W, Yang S, Li T, Yu F, Zhong W, Sun T, Wang X, Li D, Liu M, Yang Y, Zhou J
EMBO reports 2024 Mar;25(3):1055-1074

The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers.

Chen H, Wu Z, Yan Z, Chen C, Zhang Y, Wang Q, Gao Y, Ling K, Hu J, Wei Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jun;11(24):e2308820

The Immune Checkpoint Protein PD-L1 Regulates Ciliogenesis and Hedgehog Signaling.

Agborbesong E, Li X
Cells 2024 Jun 8;13(12)

Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport.

Hiyamizu S, Qiu H, Vuolo L, Stevenson NL, Shak C, Heesom KJ, Hamada Y, Tsurumi Y, Chiba S, Katoh Y, Stephens DJ, Nakayama K
Journal of cell science 2023 Mar 1;136(5)

Disease-associated mutations in WDR34 lead to diverse impacts on the assembly and function of dynein-2.

Shak C, Vuolo L, Uddin B, Katoh Y, Brown T, Mukhopadhyay AG, Heesom K, Roberts AJ, Stevenson N, Nakayama K, Stephens DJ
Journal of cell science 2023 Mar 1;136(5)

Arl13b promotes the proliferation, migration, osteogenesis, and mechanosensation of osteoblasts.

Lin T, Sun Y
Tissue & cell 2023 Jun;82:102088

Primary cilia support cartilage regeneration after injury.

Tao D, Zhang L, Ding Y, Tang N, Xu X, Li G, Niu P, Yue R, Wang X, Shen Y, Sun Y
International journal of oral science 2023 Jun 2;15(1):22

Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development.

Lee J, Kim Y, Ataliotis P, Kim HG, Kim DW, Bennett DC, Brown NA, Layman LC, Kim SH
Scientific reports 2023 Jul 29;13(1):12309

Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex.

Hiyamizu S, Qiu H, Tsurumi Y, Hamada Y, Katoh Y, Nakayama K
Biology open 2023 Jul 15;12(7)

TTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins.

Nguyen A, Goetz SC
Molecular biology of the cell 2023 Jan 1;34(1):ar8

Phosphorylation of MIF by PIP4K2a is necessary for cilia biogenesis.

Zhang L, Zhang H, Agborbesong E, Zhou JX, Li X
Cell death & disease 2023 Dec 5;14(12):795

KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease.

Rah G, Cha H, Kim J, Song J, Kim H, Oh YK, Ahn C, Kang M, Kim J, Yoo KH, Kim MJ, Ko HW, Ko JY, Park JH
Journal of the American Society of Nephrology : JASN 2022 Sep;33(9):1726-1741

LRRK1-mediated NDEL1 phosphorylation promotes cilia disassembly via dynein-2-driven retrograde intraflagellar transport.

Hanafusa H, Kedashiro S, Gotoh M, Saitoh KH, Inaba H, Nishioka T, Kaibuchi K, Inagaki M, Hisamoto N, Matsumoto K
Journal of cell science 2022 Nov 1;135(21)

The role of IFT140 in early bone healing of tooth extraction sockets.

Zhou S, Li G, Zhou T, Zhang S, Xue H, Geng J, Liu W, Sun Y
Oral diseases 2022 May;28(4):1188-1197

Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice.

Cong J, Wang X, Amiri-Yekta A, Wang L, Kherraf ZE, Liu C, Cazin C, Tang S, Hosseini SH, Tian S, Daneshipour A, Wang J, Zhou Y, Zeng Y, Yang S, He X, Li J, Cao Y, Jin L, Ray PF, Zhang F
Journal of medical genetics 2022 Jul;59(7):710-718

TTC30A and TTC30B Redundancy Protects IFT Complex B Integrity and Its Pivotal Role in Ciliogenesis.

Hoffmann F, Bolz S, Junger K, Klose F, Schubert T, Woerz F, Boldt K, Ueffing M, Beyer T
Genes 2022 Jul 1;13(7)

The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic.

Turn RE, Hu Y, Dewees SI, Devi N, East MP, Hardin KR, Khatib T, Linnert J, Wolfrum U, Lim MJ, Casanova JE, Caspary T, Kahn RA
Molecular biology of the cell 2022 Feb 1;33(2):ar13

Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E.

Dewees SI, Vargová R, Hardin KR, Turn RE, Devi S, Linnert J, Wolfrum U, Caspary T, Eliáš M, Kahn RA
Molecular biology of the cell 2022 Apr 1;33(4):ar33

ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.

Fujisawa S, Qiu H, Nozaki S, Chiba S, Katoh Y, Nakayama K
Biology open 2021 Sep 15;10(9)

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

Quidwai T, Wang J, Hall EA, Petriman NA, Leng W, Kiesel P, Wells JN, Murphy LC, Keighren MA, Marsh JA, Lorentzen E, Pigino G, Mill P
eLife 2021 Nov 4;10

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Chen C, Xu Q, Zhang Y, Davies BA, Huang Y, Katzmann DJ, Harris PC, Hu J, Ling K
Science advances 2021 Jun;7(26)

Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.

Luo M, Lin Z, Zhu T, Jin M, Meng D, He R, Cao Z, Shen Y, Lu C, Cai R, Zhao Y, Wang X, Li H, Wu S, Zou X, Luo G, Cao L, Huang M, Jiao H, Gao H, Sui R, Zhao C, Ma X, Cao M
Genetics in medicine : official journal of the American College of Medical Genetics 2021 Jun;23(6):1041-1049

Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.

Ganga AK, Kennedy MC, Oguchi ME, Gray S, Oliver KE, Knight TA, De La Cruz EM, Homma Y, Fukuda M, Breslow DK
Current biology : CB 2021 Jul 12;31(13):2895-2905.e7

Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry.

Qiu H, Fujisawa S, Nozaki S, Katoh Y, Nakayama K
Biology open 2021 Jan 25;10(1)

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Duan S, Li H, Zhang Y, Yang S, Chen Y, Qiu B, Huang C, Wang J, Li J, Zhu X, Yan X
The EMBO journal 2021 Jan 15;40(2):e105499

Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.

Kobayashi T, Ishida Y, Hirano T, Katoh Y, Nakayama K
Molecular biology of the cell 2021 Jan 1;32(1):45-56

RAB19 Directs Cortical Remodeling and Membrane Growth for Primary Ciliogenesis.

Jewett CE, Soh AWJ, Lin CH, Lu Q, Lencer E, Westlake CJ, Pearson CG, Prekeris R
Developmental cell 2021 Feb 8;56(3):325-340.e8

Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Hong JJ, Kim KE, Park SY, Bok J, Seo JT, Moon SJ
Molecules and cells 2021 Aug 31;44(8):591-601

O-GlcNAc transferase regulates centriole behavior and intraflagellar transport to promote ciliogenesis.

Yu F, Li T, Sui Y, Chen Q, Yang S, Yang J, Hong R, Li D, Yan X, Zhao W, Zhu X, Zhou J
Protein & cell 2020 Nov;11(11):852-857

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Wang W, Allard BA, Pottorf TS, Wang HH, Vivian JL, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Yan H, Chen C, Chen H, Hong H, Huang Y, Ling K, Hu J, Wei Q
Nature communications 2020 May 4;11(1):2196

A novel homozygous mutation in WDR19 induces disorganization of microtubules in sperm flagella and nonsyndromic asthenoteratospermia.

Ni X, Wang J, Lv M, Liu C, Zhong Y, Tian S, Wu H, Cheng H, Gao Y, Tan Q, Chen B, Li Q, Song B, Wei Z, Zhou P, He X, Zhang F, Cao Y
Journal of assisted reproduction and genetics 2020 Jun;37(6):1431-1439

Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF.

Lv M, Liu W, Chi W, Ni X, Wang J, Cheng H, Li WY, Yang S, Wu H, Zhang J, Gao Y, Liu C, Li C, Yang C, Tan Q, Tang D, Zhang J, Song B, Chen YJ, Li Q, Zhong Y, Zhang Z, Saiyin H, Jin L, Xu Y, Zhou P, Wei Z, Zhang C, He X, Zhang F, Cao Y
Journal of medical genetics 2020 Jul;57(7):445-453

The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis.

Yoshida S, Aoki K, Fujiwara K, Nakakura T, Kawamura A, Yamada K, Ono M, Yogosawa S, Yoshida K
eLife 2020 Aug 6;9

Novel IFT140 variants cause spermatogenic dysfunction in humans.

Wang X, Sha YW, Wang WT, Cui YQ, Chen J, Yan W, Hou XT, Mei LB, Yu CC, Wang J
Molecular genetics & genomic medicine 2019 Sep;7(9):e920

Ahi1 promotes Arl13b ciliary recruitment, regulates Arl13b stability and is required for normal cell migration.

Muñoz-Estrada J, Ferland RJ
Journal of cell science 2019 Sep 4;132(17)

Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia.

Nozaki S, Castro Araya RF, Katoh Y, Nakayama K
Biology open 2019 Sep 17;8(9)

C11ORF74 interacts with the IFT-A complex and participates in ciliary BBSome localization.

Takahara M, Kunii M, Nakamura K, Harada A, Hirano T, Katoh Y, Nakayama K
Journal of biochemistry 2019 Mar 1;165(3):257-267

The Role of IFT140 in Osteogenesis of Adult Mice Long Bone.

Tao D, Xue H, Zhang C, Li G, Sun Y
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2019 Aug;67(8):601-611

Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.

Liu W, He X, Yang S, Zouari R, Wang J, Wu H, Kherraf ZE, Liu C, Coutton C, Zhao R, Tang D, Tang S, Lv M, Fang Y, Li W, Li H, Zhao J, Wang X, Zhao S, Zhang J, Arnoult C, Jin L, Zhang Z, Ray PF, Cao Y, Zhang F
American journal of human genetics 2019 Apr 4;104(4):738-748

Cellular ciliary phenotyping indicates pathogenicity of novel variants in IFT140 and confirms a Mainzer-Saldino syndrome diagnosis.

Oud MM, Latour BL, Bakey Z, Letteboer SJ, Lugtenberg D, Wu KM, Cornelissen EAM, Yntema HG, Schmidts M, Roepman R, Bongers EMHF
Cilia 2018;7:1

A distal centriolar protein network controls organelle maturation and asymmetry.

Wang L, Failler M, Fu W, Dynlacht BD
Nature communications 2018 Sep 26;9(1):3938

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

Vuolo L, Stevenson NL, Heesom KJ, Stephens DJ
eLife 2018 Oct 16;7

Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms.

Forbes TA, Howden SE, Lawlor K, Phipson B, Maksimovic J, Hale L, Wilson S, Quinlan C, Ho G, Holman K, Bennetts B, Crawford J, Trnka P, Oshlack A, Patel C, Mallett A, Simons C, Little MH
American journal of human genetics 2018 May 3;102(5):816-831

Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.

Silva LM, Jacobs DT, Allard BA, Fields TA, Sharma M, Wallace DP, Tran PV
Scientific reports 2018 Mar 21;8(1):4985

Whole-genome sequencing in patients with ciliopathies uncovers a novel recurrent tandem duplication in IFT140.

Geoffroy V, Stoetzel C, Scheidecker S, Schaefer E, Perrault I, Bär S, Kröll A, Delbarre M, Antin M, Leuvrey AS, Henry C, Blanché H, Decker E, Kloth K, Klaus G, Mache C, Martin-Coignard D, McGinn S, Boland A, Deleuze JF, Friant S, Saunier S, Rozet JM, Bergmann C, Dollfus H, Muller J
Human mutation 2018 Jul;39(7):983-992

Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking.

Hamada Y, Tsurumi Y, Nozaki S, Katoh Y, Nakayama K
Molecular biology of the cell 2018 Jul 1;29(13):1628-1639

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.

Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, Nakayama K
Human molecular genetics 2018 Feb 1;27(3):516-528

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Bowie E, Norris R, Anderson KV, Goetz SC
PLoS genetics 2018 Dec;14(12):e1007844

Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.

Vuong LT, Iomini C, Balmer S, Esposito D, Aaronson SA, Mlodzik M
Nature communications 2018 Dec 13;9(1):5304

Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis.

Funabashi T, Katoh Y, Okazaki M, Sugawa M, Nakayama K
The Journal of cell biology 2018 Aug 6;217(8):2867-2876

Essential Role of IFT140 in Promoting Dentinogenesis.

Li G, Liu M, Zhang S, Wan H, Zhang Q, Yue R, Yan X, Wang X, Wang Z, Sun Y
Journal of dental research 2018 Apr;97(4):423-431

Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

Takei R, Katoh Y, Nakayama K
Biology open 2018 Apr 30;7(5)

In Vitro Modeling Using Ciliopathy-Patient-Derived Cells Reveals Distinct Cilia Dysfunctions Caused by CEP290 Mutations.

Shimada H, Lu Q, Insinna-Kettenhofen C, Nagashima K, English MA, Semler EM, Mahgerefteh J, Cideciyan AV, Li T, Brooks BP, Gunay-Aygun M, Jacobson SG, Cogliati T, Westlake CJ, Swaroop A
Cell reports 2017 Jul 11;20(2):384-396

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, Jackson PK
Developmental cell 2017 Jul 10;42(1):22-36.e12

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Schurmans S, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T
Cell 2017 Jan 12;168(1-2):264-279.e15

Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors.

Hirano T, Katoh Y, Nakayama K
Molecular biology of the cell 2017 Feb 1;28(3):429-439

Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E.

Nozaki S, Katoh Y, Terada M, Michisaka S, Funabashi T, Takahashi S, Kontani K, Nakayama K
Journal of cell science 2017 Feb 1;130(3):563-576

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B
Nature communications 2017 Dec 4;8(1):1928

Immunofluorescent staining of septins in primary cilia.

Kim MS, Froese CD, Xie H, Trimble WS
Methods in cell biology 2016;136:269-83

Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

Fu W, Wang L, Kim S, Li J, Dynlacht BD
Cell reports 2016 Nov 1;17(6):1505-1517

Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.

Siller SS, Burke MC, Li FQ, Takemaru K
Cell cycle (Georgetown, Tex.) 2015;14(19):3163-72

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

Xu Y, Cao J, Huang S, Feng D, Zhang W, Zhu X, Yan X
PloS one 2015;10(4):e0124378

Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly.

Ran J, Yang Y, Li D, Liu M, Zhou J
Scientific reports 2015 Aug 6;5:12917

Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.

Garcia-Gonzalo FR, Phua SC, Roberson EC, Garcia G 3rd, Abedin M, Schurmans S, Inoue T, Reiter JF
Developmental cell 2015 Aug 24;34(4):400-409

CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6.

Yang Y, Ran J, Liu M, Li D, Li Y, Shi X, Meng D, Pan J, Ou G, Aneja R, Sun SC, Zhou J
Cell research 2014 Nov;24(11):1342-53

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Martin CA, Ahmad I, Klingseisen A, Hussain MS, Bicknell LS, Leitch A, Nürnberg G, Toliat MR, Murray JE, Hunt D, Khan F, Ali Z, Tinschert S, Ding J, Keith C, Harley ME, Heyn P, Müller R, Hoffmann I, Cormier-Daire V, Dollfus H, Dupuis L, Bashamboo A, McElreavey K, Kariminejad A, Mendoza-Londono R, Moore AT, Saggar A, Schlechter C, Weleber R, Thiele H, Altmüller J, Höhne W, Hurles ME, Noegel AA, Baig SM, Nürnberg P, Jackson AP
Nature genetics 2014 Dec;46(12):1283-1292

Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.

Wei Q, Xu Q, Zhang Y, Li Y, Zhang Q, Hu Z, Harris PC, Torres VE, Ling K, Hu J
Nature communications 2013;4:2750

Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans.

Halbritter J, Bizet AA, Schmidts M, Porath JD, Braun DA, Gee HY, McInerney-Leo AM, Krug P, Filhol E, Davis EE, Airik R, Czarnecki PG, Lehman AM, Trnka P, Nitschké P, Bole-Feysot C, Schueler M, Knebelmann B, Burtey S, Szabó AJ, Tory K, Leo PJ, Gardiner B, McKenzie FA, Zankl A, Brown MA, Hartley JL, Maher ER, Li C, Leroux MR, Scambler PJ, Zhan SH, Jones SJ, Kayserili H, Tuysuz B, Moorani KN, Constantinescu A, Krantz ID, Kaplan BS, Shah JV, UK10K Consortium, Hurd TW, Doherty D, Katsanis N, Duncan EL, Otto EA, Beales PL, Mitchison HM, Saunier S, Hildebrandt F
American journal of human genetics 2013 Nov 7;93(5):915-25

Cauli: a mouse strain with an Ift140 mutation that results in a skeletal ciliopathy modelling Jeune syndrome.

Miller KA, Ah-Cann CJ, Welfare MF, Tan TY, Pope K, Caruana G, Freckmann ML, Savarirayan R, Bertram JF, Dobbie MS, Bateman JF, Farlie PG
PLoS genetics 2013 Aug;9(8):e1003746

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP( IFT140 Antibody) at dilution of 1:300
Sample type: tissue