Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-14082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STK35 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references STK35 Is Ubiquitinated by NEDD4L and Promotes Glycolysis and Inhibits Apoptosis Through Regulating the AKT Signaling Pathway, Influencing Chemoresistance of Colorectal Cancer.
Yang H, Zhu J, Wang G, Liu H, Zhou Y, Qian J
Frontiers in cell and developmental biology 2020;8:582695
Frontiers in cell and developmental biology 2020;8:582695
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STK35 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with STK35 polyclonal antibody (Product # PA5-14082) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 7 STK35 is ubiquitinated by NEDD4L and inhibits NEDD4L-mediated anti-colorectal cancer function. (A) Purification of the STK35 immunocomplex. Proteins were separated by SDS-PAGE and stained with Coomassie Blue. (B) Immunoprecipitation was performed with an IgG control, anti-STK35, or anti-NEDD4L antibody, followed by incubation with indicated antibodies. (C) Protein and mRNA expression of STK35 in SW620 cells transduced with NEDD4L-overexpressing lentivirus (NEDD4L) or blank lentivirus (Vector) in the presence of 10 muM MG132 or control (Vehicle), measured with western blot and Q-PCR. beta-Actin as the loading control. (D) NEDD4L-overexpressed SW620 cells immune-precipitated with STK35 or IgG antibody for evaluating ubiquitination. (E-I) SW620 cells were transduced with STK35-overexpressing lentivirus (STK35) after NEDD4L-overexpressing lentivirus (NEDD4L) or blank lentivirus (Vector) transfection. (E) Cell apoptosis assessed by flow cytometry and quantification accordingly. (F) Cellular LDH levels measured by biochemical analysis. (G,H) Cellular energy metabolism reflected by (G) oxygen consumption rate and (H) extracellular acidification rate. (I,J) Cellular protein levels of STK35, cleaved caspase-3, cleaved caspase-9, GLUT1, HK-2, p-AKT, and AKT, measured with western blot. beta-Actin as the loading control. *** P < 0.001, compared with Vector. ### P < 0.001, compared with NEDD4L.
