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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA5-78154 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL37 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: 293T, HeLa, Jurkat, Raji, NCI-H929 Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Chicken (100%), Chimpanzee (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPL37 in A) Jurkat whole cell lysate, B)Raji whole cell lysate, C) NCI-H929 whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with RPL37 polyclonal antibody (Product # PA5-78154) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPL37 in A) 293T whole cell lysate, B) HeLa whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with RPL37 polyclonal antibody (Product # PA5-78154) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL37 Polyclonal Antibody detects RPL37 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract.15 % SDS-PAGE. RPL37 Polyclonal Antibody (Product # PA5-78154) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL37 Polyclonal Antibody detects RPL37 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.15 % SDS-PAGE. RPL37 Polyclonal Antibody (Product # PA5-78154) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL37 Polyclonal Antibody detects RPL37 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL37 protein stained by RPL37 Polyclonal Antibody (Product # PA5-78154) diluted at 1:500. Blue: Hoechst 33342 staining.
