Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-18380 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nogo Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PA5-18380 (0.3 µg/mL) in Human Brain lysate with (B) and without (A) blocking with the immunising peptide, the primary antibody incubation was 1 hour and detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Nogo Receptor Polyclonal Antibody (Product # PA5-18380) and a 80 kDa band corresponding to Nogo receptor was observed across the cell lines and tissues tested except Mouse Skeletal Muscle, Mouse and Rat Heart. Tissue extracts of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse cerebellum (Lane 3), Rat cerebellum (Lane 4), Mouse skeletal Muscle (Lane 5), Mouse Heart (Lane 6), Rat Heart (Lane 7), whole cell extracts (30 µg lysate) of Hep G2 (Lane 8), IMR-32 (Lane 9) and SH-SY5Y (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Antibody were Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005) (Ref: 10.1007/s00018-011-0893-1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Nogo Receptor was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRKCI Rabbit Polyclonal Antibody (Product # PA5-18380) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32814) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing membrane staining of Nogo receptor in SH-SY5Y cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.