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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00084936-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00084936-M02, RRID:AB_1112628
- Product name
- ZFYVE19 monoclonal antibody (M02), clone 3G4-2B11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ZFYVE19.
- Antigen sequence
MESRCYGCAVKFTLFKKEYGCKNCGRAFCSGCLSF
SAAVPRTGNTQQKVCKQCHEVLTRGSSANASKWSP
PQNYKKRVAALEAKQKPSTSQSQGLTRQDQMIAER
LARLRQENKPKLVPSQAEIEARLAALKDERQGSIP
STQEMEARLAALQGRVLPSQTPQPAHHTPDTRTQA
QQTQDLLTQLAAEVAIDESWKGGGPAASLQNDLNQ
GGPGSTNSKRQANWSLEEEKSRLLAEAALELREEN
TRQERILALAKRLAMLRGQDPERVTLQDYRLPDSD
DDEDEETAIQRVLQQLTEEAALDEASGFNIPAEQA
SRPWTQPRGAEPEAQDVDPRPEAEEEELPWCCICN
EDATLRCAGCDGDLFCARCFREGHDAFELKEHQTS
AYSPPRAGQEH- Isotype
- IgG
- Antibody clone number
- 3G4-2B11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ZFYVE19 expression in transfected 293T cell line by ZFYVE19 monoclonal antibody (M02), clone 3G4-2B11.Lane 1: ZFYVE19 transfected lysate (Predicted MW: 36.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ZFYVE19 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ZFYVE19 transfected lysate using anti-ZFYVE19 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ZFYVE19 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
