PA5-116844

antibody from Invitrogen Antibodies

Targeting: CELF5 BRUNOL5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116844

Provider

Invitrogen Antibodies

Product name

BRUNOL5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: U-251MG, U-87MG, Mouse testis Immunogen sequence: MARLTESEAR RQQQQLLQPR PSPVGSSGPE PPGGQPDGMK DLDAI

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.63 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRUNOL5 in extracts of various cell lines. Samples were incubated with BRUNOL5 Polyclonal antibody (Product # PA5-116844) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of BRUNOL5 in L929 cells using BRUNOL5 Polyclonal Antibody (Product # PA5-116844) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of BRUNOL5 in L929 cells. Samples were incubated with BRUNOL5 Polyclonal antibody (Product # PA5-116844) using a dilution of 1:100. Blue: DAPI for nuclear staining.