PA5-109295

antibody from Invitrogen Antibodies

Targeting: NFIC CTF, CTF5, NF-I, NFI

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109295

Provider

Invitrogen Antibodies

Product name

NFIC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, SiHa, A431.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nfic in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nfic polyclonal antibody (Product # PA5-109295), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nfic in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nfic polyclonal antibody (Product # PA5-109295), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nfic in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nfic polyclonal antibody (Product # PA5-109295), followed by DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Nfic in SiHa cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nfic polyclonal antibody (Product # PA5-109295) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.