Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-81102 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thioredoxin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Thioredoxin 2 in Lane A: HeLa Whole Cell Lysate (30 µg), Lane B: 293 Whole Cell Lysate (30 µg). Samples were probed using a Thioredoxin 2 Polyclonal Antibody (Product # PA5-81102) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), HRP Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:18 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Thioredoxin 2 Polyclonal Antibody (Product # PA5-81102) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: 293 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Thioredoxin 2 in HELA cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Thioredoxin 2 Polyclonal Antibody (Product # PA5-81102, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Thioredoxin 2 in human kidney with Thioredoxin 2 Polyclonal Antibody (Product # PA5-81102, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Thioredoxin 2 in human small intestine with Thioredoxin 2 Polyclonal Antibody (Product # PA5-81102, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thioredoxin 2 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 µL with Thioredoxin 2 Polyclonal Antibody (Product # PA5-81102) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Thioredoxin 2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 43 kDa.