Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
- Other assay [1]
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Validation data
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- Product number
- MA5-26311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHAC1 Monoclonal Antibody (OTI1E2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1E2
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.
Xu Y, Zhang N, Chen C, Xu X, Luo A, Yan Y, Lu Y, Liu J, Ou X, Tan Y, Liang Y, Chen L, Song X, Liu X
Frontiers in oncology 2022;12:859621
Frontiers in oncology 2022;12:859621
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHAC1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with CHAC1 (Product # MA5-26311) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CHAC1 in COS7 cells. Cells were transfected with a plasmid overexpressing CHAC1 and probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Induction of ferroptosis reversed the effects of ATF4 suppression on Sev-induced ferroptosis in glioma cells. Ferroptosis inducer Erastin was used to incubate in Sev-treated and ATF4-suppressed U87 and U251 cells. Cell viability was detected using CCK-8 assay in U87 (A) and U251 cells (B) . Fe2+ concentrations were determined by colorimetric assay in U87 and U251 cells (C) . ROS assay combined with flow cytometry was used to observe the content of ROS generation in U87 and U251 cells (D) ; the ratio of ROS generation was calculated (E) . The expression of ATF4 and CHAC1 in U87 and U251 cells was detected using Western blotting; GAPDH was used as the internal control (F, G) . Data were expressed as mean +- standard deviation. *p < 0.05, **p < 0.01, compared with control.