15207-1-AP

antibody from Proteintech Group

Targeting: CHAC1 MGC4504

Provider product page for 15207-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
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Product number: 15207-1-AP - Provider product page
Provider: Proteintech Group
Product name: CHAC1 antibody
Antibody type: Polyclonal
Description: KD/KO validated CHAC1 antibody (Cat. #15207-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, hamster and has been validated for the following applications: IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat, Hamster
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CHAC1 protein structure - 15207-1-AP shown in red.

Submitted references Amino acid restriction sensitizes lung cancer cells to ferroptosis via GCN2-dependent activation of the integrated stress response.

Garellick VA, Gul N, Horrieh P, Mustafa D, Patel AAH, Dankis M, Alvarez SW, Berndtson J, Mahdavi S, Schwarz M, Persson A, Zahirovic F, Wiel C, Sayin VI, Lindahl P
Redox biology 2026 Mar;90:103988

Dissecting Complex Interactions Between Ferroptosis and the Proteasome.

Murray MB, Upadhyay R, Szylo K, Gautam A, Goncalves J, Forcina G, Vinayak A, Brandmann O, Dixon SJ
bioRxiv : the preprint server for biology 2026 Mar 30;

HIF-mediated regulation of glutathione-specific γ-glutamyl cyclotransferase 1 contributes to tubular cell death in renal ischemia-reperfusion injury.

Kihira Y, Homma T, Matsunaga S, Yamaguchi K, Tsounapi P, Yamaguchi T, Fujimura Y, Sato E, Tamaki T, Tomita S
American journal of physiology. Renal physiology 2026 Jun 1;330(6):F653-F664

Mechanism of Saikosaponin D in regulating ferroptosis in patient-derived lung adenocarcinoma organoids via upregulation of ATF3/CHOP/CHAC1 signaling.

Li J, Ma Q, Huang C, Zeng X, He J, You L, Zhuang X, Xiao P, You F, Ren Y, Zheng C
Scientific reports 2026 Jan 31;16(1):2513

NUPR1 alleviates renal ischemia reperfusion injury by inhibiting ferroptosis involving the ATF4-CHAC1 pathway.

Luo Y, Han J, Huang G
Cellular signalling 2026 Aug;144:112547

Febuxostat therapy improved the outcomes of cardiorenal syndrome rodent through alleviating xanthine oxidase-induced oxidative stress and mitochondrial dysfunction.

Yang CC, Yue Y, Chen YL, Chiang JY, Wang YT, Huang CR, Cheng BC, Hsu TW, Yip HK
International journal of biological sciences 2025;21(4):1749-1766

Shengqing Jiangzhuo Capsule Alleviates Intestinal Inflammation in Chronic Kidney Disease by Downregulating CHAC1 to Inactivate the HIF-1 Pathway.

Li Z, Wang J, Lin Y, Zhang S, Zhang L, Wu X, Wang C
Mediators of inflammation 2025;2025:2173234

Dot1l Regulates the Spontaneous Bone Regeneration of Periosteum-Derived Stem Cells by Regulating Chac1 Expression.

Jiang T, Fang B, Yu Z, Cao D
Stem cells international 2025;2025:1508850

miR-210-3p Ameliorates Metabolic Dysfunction-Associated Steatohepatitis-Related Fibrosis by Targeting ISCU and Induces Ferroptosis.

Dai Z, Sun Z, Chen Q, Li Y, Sun Y, Jiao M, Yin X, Liu R, Liang H, Zhu C
Liver international : official journal of the International Association for the Study of the Liver 2025 Sep;45(9):e70282

Unraveling the molecular mechanisms of paraquat toxicity: The interplay of ATF4, ferroptosis, and endoplasmic reticulum stress.

Ke J, Cai Q, Ge X, Zhang S
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2025 Sep;203:115602

ChaC1-based drug screenings identify a synergistic lethal effect of auranofin and proteasome inhibitors in hepatocellular carcinoma cells.

Yu C, Liu J, Jian H, Wen B, Jiang M, Zhang B, Lin M, Lin J, Dai Z, Zhong CQ, Zheng M
Cell death discovery 2025 Nov 17;11(1):532

The PERK-eIF2α-ATF4 Axis Is Involved in Mediating ER-Stress-Induced Ferroptosis via DDIT4-mTORC1 Inhibition and Acetaminophen-Induced Hepatotoxicity.

Nghiem TT, Nguyen KA, Kusuma F, Park S, Park J, Joe Y, Han J, Chung HT
Antioxidants (Basel, Switzerland) 2025 Mar 3;14(3)

Vitamin D3 and its active form calcitriol suppress erythroleukemia through upregulation of CHAC1 and downregulation of NOTCH1.

Hong J, Yang Z, Gao J, Yu K, Hu A, Kuang Y, Gajendran B, Zacksenhaus E, Xiao X, Wang C, Liu W, Ben-David Y
Medical oncology (Northwood, London, England) 2025 Mar 27;42(5):138

Protein S-glutathionylation confers cellular resistance to ferroptosis induced by glutathione depletion.

Ju Y, Zhang Y, Tian X, Zhu N, Zheng Y, Qiao Y, Yang T, Niu B, Li X, Yu L, Liu Z, Wu Y, Zhi Y, Dong Y, Xu Q, Yang X, Wang X, Wang X, Deng H, Mao Y, Li X
Redox biology 2025 Jun;83:103660

Astragalin promotes HSCs ferroptosis through NCOA4 mediated ferritinophagy to alleviate liver fibrosis in zebrafish and mice.

Wang Y, Kuang S, Li K, Chen S, Yang M, Deng K, Li M, Xie S, Chen Q, Wen J, Zhou C, Cheng W, Huang S, Lv Z
Communications biology 2025 Jul 21;8(1):1081

TEC-mediated tRF-31R9J regulates histone lactylation and acetylation by HDAC1 to suppress hepatocyte ferroptosis and improve non-alcoholic steatohepatitis.

Zhu J, Wu X, Mu M, Zhang Q, Zhao X
Clinical epigenetics 2025 Jan 21;17(1):9

E7386 Enhances Lenvatinib's Antitumor Activity in Preclinical Models and Human Hepatocellular Carcinoma.

Mesropian A, Gris-Oliver A, Balaseviciute U, Potdar AA, Kimura T, Shen J, Torres-Marcén M, Abril-Fornaguera J, Piqué-Gili M, Camell-Raventos D, Peix J, Fernández-Martínez E, Huguet-Pradell J, Hernández de Sande A, Keraite I, Esteban-Fabró R, Bárcena-Varela M, Lindblad KE, Lujambio A, Guccione E, Thung S, Ikeda M, Kudo M, Sia D, Pinyol R, Llovet JM
Clinical cancer research : an official journal of the American Association for Cancer Research 2025 Dec 1;31(23):5037-5050

Juglone induces ferroptotic effect on hepatocellular carcinoma and pan-cancer via the FOSL1-HMOX1 axis.

Wang C, Zhao Y, Peng Y, Chen W, Zhu J, Gu C, Huo R, Ding L, Liu Y, Liu T, Zhang C, Yang W, Wang H, Guo W, Wang B
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Apr;139:156417

Prognostic Values of Ferroptosis-Related Proteins ACSL4, SLC7A11, and CHAC1 in Cholangiocarcinoma.

Amontailak S, Titapun A, Jusakul A, Thanan R, Kimawaha P, Jamnongkan W, Thanee M, Sirithawat P, Techasen A
Biomedicines 2024 Sep 13;12(9)

SGLT2 inhibitor downregulated oxidative stress via activating AMPK pathway for cardiorenal (CR) protection in CR syndrome rodent fed with high protein diet.

Yang CC, Chen KH, Yue Y, Cheng BC, Hsu TW, Chiang JY, Chen CH, Liu F, Xiao J, Yip HK
Journal of molecular histology 2024 Oct;55(5):803-823

MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma.

Wanbiao Z, Jing M, Shi Z, Tengxiang C, Xueke Z, Haiyang L
Molecular and cellular biochemistry 2024 Oct;479(10):2769-2784

CHAC1 blockade suppresses progression of lung adenocarcinoma by interfering with glucose metabolism via hijacking PKM2 nuclear translocation.

Pan J, Wu S, Pan Q, Zhang Y, He L, Yao Q, Chen J, Li J, Xu Y
Cell death & disease 2024 Oct 5;15(10):728

Methionine-SAM metabolism-dependent ubiquinone synthesis is crucial for ROS accumulation in ferroptosis induction.

Xia C, Peng P, Zhang W, Xing X, Jin X, Du J, Peng W, Hao F, Zhao Z, Dong K, Tian M, Feng Y, Ba X, Wei M, Wang Y
Nature communications 2024 Oct 17;15(1):8971

Cynarin alleviates acetaminophen-induced acute liver injury through the activation of Keap1/Nrf2-mediated lipid peroxidation defense via the AMPK/SIRT3 signaling pathway.

Zhao L, Zhang X, Chen Z, Lai Y, Xu J, Zhou R, Ma P, Cai W, Zeng Y, Wu X, Ying H, Yu F
Food & function 2024 May 7;15(9):4954-4969

Novel insights into the protective effects of leonurine against acute kidney injury: Inhibition of ER stress-associated ferroptosis via regulating ATF4/CHOP/ACSL4 pathway.

Cheng R, Wang X, Huang L, Lu Z, Wu A, Guo S, Li C, Mao W, Xie Y, Xu P, Tian R
Chemico-biological interactions 2024 May 25;395:111016

Whole transcriptome profiling reveals a lncMDP1 that regulates myogenesis by adsorbing miR-301a-5p targeting CHAC1.

Chen B, Cai H, Niu Y, Zhang Y, Wang Y, Liu Y, Han R, Liu X, Kang X, Li Z
Communications biology 2024 May 2;7(1):518

Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC.

Ward NP, Yoon SJ, Flynn T, Sherwood AM, Olley MA, Madej J, DeNicola GM
Nature communications 2024 May 18;15(1):4244

Brusatol hinders the progression of bladder cancer by Chac1/Nrf2/SLC7A11 pathway.

Yu X, He Z, Wang Z, Ke S, Wang H, Wang Q, Li S
Experimental cell research 2024 May 15;438(2):114053

Cancer associated fibroblast secreted miR-432-5p targets CHAC1 to inhibit ferroptosis and promote acquired chemoresistance in prostate cancer.

Zhao J, Shen J, Mao L, Yang T, Liu J, Hongbin S
Oncogene 2024 Jun;43(27):2104-2114

ChaC1 upregulation reflects poor prognosis in a variety of cancers: analysis of the major missense SNPs of ChaC1 as an aid to refining prognosis.

Parande D, Suyal S, Bachhawat AK
Gene 2024 Jun 30;913:148386

Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer.

Fan P, Wang J, Li R, Chang K, Liu L, Wang Y, Wang Z, Zhang B, Ji C, Zhang J, Chen S, Ling R
Frontiers in oncology 2023;13:1178595

Autoregulatory control of mitochondrial glutathione homeostasis.

Liu Y, Liu S, Tomar A, Yen FS, Unlu G, Ropek N, Weber RA, Wang Y, Khan A, Gad M, Peng J, Terzi E, Alwaseem H, Pagano AE, Heissel S, Molina H, Allwein B, Kenny TC, Possemato RL, Zhao L, Hite RK, Vinogradova EV, Mansy SS, Birsoy K
Science (New York, N.Y.) 2023 Nov 17;382(6672):820-828

p53 Activates the Lipoxygenase Activity of ALOX15B via Inhibiting SLC7A11 to Induce Ferroptosis in Bladder Cancer Cells.

Li X, Xiong W, Wang Y, Li Y, Cheng X, Liu W
Laboratory investigation; a journal of technical methods and pathology 2023 May;103(5):100058

Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts.

Crabtree AA, Boehnke N, Bates FS, Hackel BJ
Proceedings of the National Academy of Sciences of the United States of America 2023 May 2;120(18):e2219885120

Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure.

Yuan Y, Mei Z, Qu Z, Li G, Yu S, Liu Y, Liu K, Shen Z, Pu J, Wang Y, Wang C, Sun Z, Liu Q, Pang X, Wang A, Ren Z, Wang T, Liu Y, Hong J, Xie J, Li X, Wang Z, Du W, Yang B
Signal transduction and targeted therapy 2023 Mar 27;8(1):121

Anti-CHAC1 exosomes for nose-to-brain delivery of miR-760-3p in cerebral ischemia/reperfusion injury mice inhibiting neuron ferroptosis.

Wang Y, Niu H, Li L, Han J, Liu Z, Chu M, Sha X, Zhao J
Journal of nanobiotechnology 2023 Mar 27;21(1):109

Treatment with paraquat affects the expression of ferroptosis-related genes.

Ge X, Cai Q, Zhang S, Wu X, Ying P, Ke J, Yang Z
Human & experimental toxicology 2023 Jan-Dec;42:9603271231167585

Dual inhibition of thioredoxin reductase and proteasome is required for auranofin-induced paraptosis in breast cancer cells.

Seo MJ, Kim IY, Lee DM, Park YJ, Cho MY, Jin HJ, Choi KS
Cell death & disease 2023 Jan 19;14(1):42

CHAC1 as a Novel Contributor of Ferroptosis in Retinal Pigment Epithelial Cells with Oxidative Damage.

Liu Y, Wu D, Fu Q, Hao S, Gu Y, Zhao W, Chen S, Sheng F, Xu Y, Chen Z, Yao K
International journal of molecular sciences 2023 Jan 13;24(2)

ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis.

Yang M, Chen Y, Huang X, Shen F, Meng Y
Lung 2023 Aug;201(4):425-441

Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade.

Xue Y, Lu F, Chang Z, Li J, Gao Y, Zhou J, Luo Y, Lai Y, Cao S, Li X, Zhou Y, Li Y, Tan Z, Cheng X, Li X, Chen J, Wang W
Nature communications 2023 Aug 8;14(1):4758

Salidroside ameliorates acetaminophen-induced acute liver injury through the inhibition of endoplasmic reticulum stress-mediated ferroptosis by activating the AMPK/SIRT1 pathway.

Xu J, Zhao L, Zhang X, Ying K, Zhou R, Cai W, Wu X, Jiang H, Xu Q, Miao D, Zeng Y, Yu F
Ecotoxicology and environmental safety 2023 Aug 7;262:115331

Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.

Huang S, Wang Y, Xie S, Lai Y, Mo C, Zeng T, Kuang S, Deng G, Zhou C, Chen Y, Huang S, Gao L, Lv Z
Cellular and molecular gastroenterology and hepatology 2022;13(6):1649-1672

DJ-1 inhibits glutathione degradation by downregulating CHAC1 expression in astrocytes.

Ge Y, Zheng X, Mao S, Zhang Q, Hu G, Wei Y
Neuroscience research 2022 Nov;184:62-69

Glaucocalyxin A impairs tumor growth via amplification of the ATF4/CHOP/CHAC1 cascade in human oral squamous cell carcinoma.

Wang X, He MJ, Chen XJ, Bai YT, Zhou G
Journal of ethnopharmacology 2022 May 23;290:115100

Xiao R, Wang S, Guo J, Liu S, Ding A, Wang G, Li W, Zhang Y, Bian X, Zhao S, Qiu W
Journal of cellular and molecular medicine 2022 Feb;26(4):1183-1193

Differences in cell death in methionine versus cysteine depletion.

Wallis KF, Morehead LC, Bird JT, Byrum SD, Miousse IR
Environmental and molecular mutagenesis 2021 Mar;62(3):216-226

Metabolomic analysis of spontaneous neutrophil apoptosis reveals the potential involvement of glutathione depletion.

Yuyun X, Fan Y, Weiping W, Qing Y, Bingwei S
Innate immunity 2021 Jan;27(1):31-40

High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway.

Zhang X, Li W, Ma Y, Zhao X, He L, Sun P, Wang H
Free radical biology & medicine 2021 Dec;177:156-166

Li D, Liu S, Xu J, Chen L, Xu C, Chen F, Xu Z, Zhang Y, Xia S, Shao Y, Wang Y
Journal of cellular and molecular medicine 2021 Apr;25(7):3610-3621

Xylitol acts as an anticancer monosaccharide to induce selective cancer death via regulation of the glutathione level.

Tomonobu N, Komalasari NLGY, Sumardika IW, Jiang F, Chen Y, Yamamoto KI, Kinoshita R, Murata H, Inoue Y, Sakaguchi M
Chemico-biological interactions 2020 Jun 1;324:109085

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