PA5-31231

antibody from Invitrogen Antibodies

Targeting: RBPMS HERMES

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31231

Provider

Invitrogen Antibodies

Product name

RBPMS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, mouse heart. Predicted reactivity: Mouse (99%), Rat (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.55 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Genetic inhibition of collapsin response mediator protein-2 phosphorylation ameliorates retinal ganglion cell death in normal-tension glaucoma models.

Brahma MM, Takahashi K, Namekata K, Harada T, Goshima Y, Ohshima T
Genes to cells : devoted to molecular & cellular mechanisms 2022 Aug;27(8):526-536

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Genetic inhibition of CRMP2 phosphorylation at serine 522 promotes axonal regeneration after optic nerve injury.

Kondo S, Takahashi K, Kinoshita Y, Nagai J, Wakatsuki S, Araki T, Goshima Y, Ohshima T
Scientific reports 2019 May 10;9(1):7188

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBPMS using 30 µg of A549 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RBPMS polyclonal antibody (Product # PA5-31231) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBPMS using 50 µg of mouse heart lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RBPMS polyclonal antibody (Product # PA5-31231) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBPMS was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RBPMS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RBPMS Polyclonal Antibody (Product # PA5-31231). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS Polyclonal Antibody (Product # PA5-31231) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RBPMS was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RBPMS Polyclonal Antibody (Product # PA5-31231). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS Polyclonal Antibody (Product # PA5-31231) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RBPMS was performed in A549 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RBPMS Polyclonal Antibody (Product # PA5-31231) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1 FIGURE Production of p25 after N-methyl-D-aspartate (NMDA) injection. (a, b) Dose-dependent decrease in the number of retinal ganglion cells (RGCs) observed 1 week after the injection of phosphate-buffered saline (PBS) or NMDA in 5 or 20 nmol concentrations. The retina samples are stained with anti-RNA-binding protein with multiple splicing, a specific marker for RGCs (Rodriguez et al., 2015). (c) Time course of p25 production within 24 h and amounts of p35 and p25 in retina samples from PBS or 20 nmol NMDA injected in the eye indicated time points by western blot analysis. (d) Quantification of p25/p35+p25 level ( n = 4, *** p < .001; * p < .05)