Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1718 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat CLEC-2/CLEC1B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat CLEC-2/CLEC1B in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CLEC-1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAF36777
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice.
Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides.
Clinicopathological correlations of podoplanin (gp38) expression in rheumatoid synovium and its potential contribution to fibroblast platelet crosstalk.
The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade.
DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets.
Milasan A, Jean G, Dallaire F, Tardif JC, Merhi Y, Sorci-Thomas M, Martel C
Journal of the American Heart Association 2017 Sep 22;6(9)
Journal of the American Heart Association 2017 Sep 22;6(9)
Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides.
Delierneux C, Donis N, Servais L, Wéra O, Lecut C, Vandereyken M, Musumeci L, Rahmouni S, Schneider J, Eble JA, Lancellotti P, Oury C
Journal of thrombosis and haemostasis : JTH 2017 May;15(5):983-997
Journal of thrombosis and haemostasis : JTH 2017 May;15(5):983-997
Clinicopathological correlations of podoplanin (gp38) expression in rheumatoid synovium and its potential contribution to fibroblast platelet crosstalk.
Del Rey MJ, Faré R, Izquierdo E, Usategui A, Rodríguez-Fernández JL, Suárez-Fueyo A, Cañete JD, Pablos JL
PloS one 2014;9(6):e99607
PloS one 2014;9(6):e99607
The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade.
Fuller GL, Williams JA, Tomlinson MG, Eble JA, Hanna SL, Pöhlmann S, Suzuki-Inoue K, Ozaki Y, Watson SP, Pearce AC
The Journal of biological chemistry 2007 Apr 27;282(17):12397-409
The Journal of biological chemistry 2007 Apr 27;282(17):12397-409
DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets.
Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pöhlmann S
Journal of virology 2006 Sep;80(18):8951-60
Journal of virology 2006 Sep;80(18):8951-60
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat CLEC-2/CLEC1B by Western Blot. Western blot shows lysates of mouse kidney tissue, rat kidney tissue, and mouse bone marrow. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CLEC-2/CLEC1B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CLEC-2/CLEC1B by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for CLEC-2/CLEC1B at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.