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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Product number
- NBP2-50105 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Apc4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hyperphosphorylation of CDH1 in Glioblastoma Cancer Stem Cells Attenuates APC/CCDH1 Activity and Pharmacologic Inhibition of APC/CCDH1/CDC20 Compromises Viability.
De K, Grubb TM, Zalenski AA, Pfaff KE, Pal D, Majumder S, Summers MK, Venere M
Molecular cancer research : MCR 2019 Jul;17(7):1519-1530
Molecular cancer research : MCR 2019 Jul;17(7):1519-1530
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