Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-62091 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOP56 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EDPSISFSKP KKKKSFSKEE LMSSDLEETA GSTS Highest antigen sequence identity to the following orthologs: Mouse - 59%, Rat - 62%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP56 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a NOP56 Polyclonal Antibody (Product # PA5-62091).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP56 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a NOP56 Polyclonal Antibody (Product # PA5-62091). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NOP56 in human cell line U-2 OS shows positivity in nucleoli. Samples were probed using a NOP56 Polyclonal Antibody (Product # PA5-62091).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOP56 in human fallopian tube and pancreas tissues using NOP56 Polyclonal Antibody (Product # PA5-62091). Corresponding NOP56 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOP56 in human fallopian tube using NOP56 Polyclonal Antibody (Product # PA5-62091) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOP56 in human pancreas using NOP56 Polyclonal Antibody (Product # PA5-62091) shows low expression as expected.