Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-46848 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46848, RRID:AB_10009911
- Product name
- Rabbit Polyclonal NOP56 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOP56 Antibody [NBP1-46848] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP56 antibody NBP1-46848 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP56 was also immunoprecipitated by rabbit anti-NOP56 antibody NBP1-46847, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NOP56 Antibody [NBP1-46848] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NOP56 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB