Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1211 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Growth Hormone R/GHR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat Growth Hormone R/GHR in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse GHR and recombinant human GHR is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16310
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody. Recombinant Rat Growth Hormone R/GHR Fc Chimera (Catalog # 1211-GR) inhibits Recombinant Human Growth Hormone (Catalog # 1067-GH) induced proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human Growth Hormone (0.2 ng/mL) activity elicited by Recombinant Rat Growth Hormone R/GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Rat Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1211). The ND50 is typically 0.075-0.3 µg/mL.