Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF1210 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Growth Hormone R/GHR Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Growth Hormone R/GHR in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse GHR and recombinant rat GHR is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P10912
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Growth Hormone R/GHR in Human Breast Cancer Tissue. Growth Hormone R/GHR was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Growth Hormone R/GHR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1210) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.