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- Antibody Data
- Antigen structure
- References [4]
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- Flow cytometry [1]
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- Product number
- MAB67761 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CLEC9a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CLEC9a.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates.
Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas.
Boosting antibody responses by targeting antigens to dendritic cells.
Efficient and versatile manipulation of the peripheral CD4+ T-cell compartment by antigen targeting to DNGR-1/CLEC9A.
Li J, Ahmet F, Sullivan LC, Brooks AG, Kent SJ, De Rose R, Salazar AM, Reis e Sousa C, Shortman K, Lahoud MH, Heath WR, Caminschi I
European journal of immunology 2015 Mar;45(3):854-64
European journal of immunology 2015 Mar;45(3):854-64
Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas.
Picco G, Beatson R, Taylor-Papadimitriou J, Burchell JM
European journal of immunology 2014 Jul;44(7):1947-55
European journal of immunology 2014 Jul;44(7):1947-55
Boosting antibody responses by targeting antigens to dendritic cells.
Caminschi I, Shortman K
Trends in immunology 2012 Feb;33(2):71-7
Trends in immunology 2012 Feb;33(2):71-7
Efficient and versatile manipulation of the peripheral CD4+ T-cell compartment by antigen targeting to DNGR-1/CLEC9A.
Joffre OP, Sancho D, Zelenay S, Keller AM, Reis e Sousa C
European journal of immunology 2010 May;40(5):1255-65
European journal of immunology 2010 May;40(5):1255-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CLEC9a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Anti-Mouse Integrin alpha X/CD11c PE-conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67761) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
