AF6776

antibody from Novus Biologicals

Targeting: CLEC9A CD370, DNGR-1, HEEE9341, UNQ9341

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6776

Provider

Novus Biologicals

Product name

Sheep Polyclonal CLEC9a Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse CLEC9a in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CLEC9a, rhCLEC2, and rhCLEC12B is observed.

Reactivity

Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Cross-talk between iNKT cells and CD8 T cells in the spleen requires the IL-4/CCL17 axis for the generation of short-lived effector cells.

Valente M, Dölen Y, van Dinther E, Vimeux L, Fallet M, Feuillet V, Figdor CG
Proceedings of the National Academy of Sciences of the United States of America 2019 Dec 17;116(51):25816-25827

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

CLEC9a in Mouse Spleen. CLEC9a was detected in perfusion fixed frozen sections of mouse spleen using Sheep Anti-Mouse CLEC9a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6776) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.