353805

antibody from BioLegend

Targeting: CLEC9A CD370, DNGR-1, HEEE9341, UNQ9341

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

353805

Provider

BioLegend

Proper citation

BioLegend Cat#353805, RRID:AB_2565518

Product name

APC anti-human CD370 (CLEC9A/DNGR1)

Antibody type

Monoclonal

Antigen

Other

Description

CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16^- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antibody clone number

8F9

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 PE/Cy7, and CLEC9A (clone 8F9) APC (top) or mouse IgG2a, κ APC isotype control (bottom). Data was gated on the CD3-negative, HLA-DR-positive monocyte population.

Conjugate

Red dye