Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 353806 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#353806, RRID:AB_2565519
- Product name
- APC anti-human CD370 (CLEC9A/DNGR1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antibody clone number
- 8F9
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 PE/Cy7, and CLEC9A (clone 8F9) APC (top) or mouse IgG2a, κ APC isotype control (bottom). Data was gated on the CD3-negative, HLA-DR-positive monocyte population.
- Conjugate
- Red dye