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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- 12-3709-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD370 (Clec9A) Monoclonal Antibody (9A11), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This monoclonal antibody 9A11 reacts with human CD370, also known as C-type lectin family member 9A (Clec9A) and DNGR1. This type II transmembrane glycoprotein is present at the cell surface in the form of a homodimer. CD370 is a cellular receptor expressed by myeloid cells that recognizes F-actin, a dead cell-associated antigen that is exposed in necrotic cells. Upon ligation, CD370 recruits Syk kinase to the ITAM-like motif in its intracellular tail and facilitates endocytosis of antigenic material that is subsequently processed and cross-presented to CD8+ T cells. Human CD370 is expressed by CD141+ (BDCA3+) dendritic cells and, to a lesser degree, by CD14+ CD16- monocytes. Unlike in mice, human plasmacytoid dendritic cells do not express CD370.
- Conjugate
- Yellow dye
- Antibody clone number
- 9A11
- Concentration
- 5 µL/Test
Submitted references Repression of phagocytosis by human CD33 is not conserved with mouse CD33.
FcαRI co-stimulation converts human intestinal CD103(+) dendritic cells into pro-inflammatory cells through glycolytic reprogramming.
Bhattacherjee A, Rodrigues E, Jung J, Luzentales-Simpson M, Enterina JR, Galleguillos D, St Laurent CD, Nakhaei-Nejad M, Fuchsberger FF, Streith L, Wang Q, Kawasaki N, Duan S, Bains A, Paulson JC, Rademacher C, Giuliani F, Sipione S, Macauley MS
Communications biology 2019;2:450
Communications biology 2019;2:450
FcαRI co-stimulation converts human intestinal CD103(+) dendritic cells into pro-inflammatory cells through glycolytic reprogramming.
Hansen IS, Krabbendam L, Bernink JH, Loayza-Puch F, Hoepel W, van Burgsteden JA, Kuijper EC, Buskens CJ, Bemelman WA, Zaat SAJ, Agami R, Vidarsson G, van den Brink GR, de Jong EC, Wildenberg ME, Baeten DLP, Everts B, den Dunnen J
Nature communications 2018 Feb 28;9(1):863
Nature communications 2018 Feb 28;9(1):863
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD141 APC (Product # 17-1419-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD370 (Clec9A) PE (right). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye
