353802

antibody from BioLegend

Targeting: CLEC9A CD370, DNGR-1, HEEE9341, UNQ9341

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

353802

Provider

BioLegend

Proper citation

BioLegend Cat#353802, RRID:AB_10983070

Product name

Purified anti-human CLEC9A (DNGR1)

Antibody type

Monoclonal

Antigen

CLEC9A

Description

CLECA, also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 APC, and CLEC9a (clone 8F9) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data was analyzed with CD3-negative and HLA-DR-positive cell population.

Conjugate

Unconjugated