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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [2]
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- Product number
- FAB6049A - Provider product page
- Provider
- R&D Systems
- Product name
- Human CLEC9a APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CLEC9a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
Q6UXN8- Isotype
- IgG
- Antibody clone number
- 683409
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references TLR3-responsive, XCR1+, CD141(BDCA-3)+/CD8α+-equivalent dendritic cells uncovered in healthy and simian immunodeficiency virus-infected rhesus macaques.
Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
Dutertre CA, Jourdain JP, Rancez M, Amraoui S, Fossum E, Bogen B, Sanchez C, Couëdel-Courteille A, Richard Y, Dalod M, Feuillet V, Cheynier R, Hosmalin A
Journal of immunology (Baltimore, Md. : 1950) 2014 May 15;192(10):4697-708
Journal of immunology (Baltimore, Md. : 1950) 2014 May 15;192(10):4697-708
Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
Balan S, Ollion V, Colletti N, Chelbi R, Montanana-Sanchis F, Liu H, Vu Manh TP, Sanchez C, Savoret J, Perrot I, Doffin AC, Fossum E, Bechlian D, Chabannon C, Bogen B, Asselin-Paturel C, Shaw M, Soos T, Caux C, Valladeau-Guilemond J, Dalod M
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CLEC9a in HEK293 Human Cell Line Transfected with Human CLEC9a and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CLEC9a and eGFP was stained with either (A) Mouse Anti-Human CLEC9a APC-conjugated Monoclonal Antibody (Catalog # FAB6049A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CLEC9a in Human Peripheral Blood Cells by Flow Cytometry. Human peripheral blood cells gated on CD3-CD141+ cells were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) and either (A) Mouse Anti-Human CLEC9a APC-conjugated Monoclonal Antibody (Catalog # FAB6049A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
