LS-B15197

antibody from LSBio

Targeting: SGCA A2, adhalin, ADL, DMDA2, LGMD2D, SCARMD1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B15197

Provider

LSBio

Product name

IHC-plus™ SGCA / DAG2 Antibody LS-B15197

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: SGCA antibody at 0.5 ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: HepG-2 whole cell lysate. Lane 3: HeLa whole cell lysate. Lane 4: 293T whole cell lysate. Lane 5: MCF7 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: SGCA antibody at 0.5µg/ml Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: 293T whole cell lysate Lane 5: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 30 kDa Observed band size: 43 kDa

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using antibody at dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using antibody at dilution of 1:100.