PA5-43065

antibody from Invitrogen Antibodies

Targeting: ZNF131 pHZ-10, ZBTB35

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-43065

Provider

Invitrogen Antibodies

Product name

ZNF131 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: DLLQDSQVHD SHMSELPEQV QVSYLEVGRI QTEEGTEVHV EELHVERVNQ Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

LINC00470 accelerates the proliferation and metastasis of melanoma through promoting APEX1 expression.

Huang T, Wang YJ, Huang MT, Guo Y, Yang LC, Liu XJ, Tan WY, Long JH
Cell death & disease 2021 Apr 19;12(5):410

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 LINC00470 activated ZNF131 to inhibit the transcription of APEX1. A The interaction of LINC00470 and ZNF131 was detected by RIP assay in normal and melanoma tissues. B , C RIP assay showed the enrichment of LINC00470 and ZNF131 in melanoma cells or the AR-overexpressed melanoma cells. D RNA pull-down and western blot analysis after the cells were transfected with OE-NC and OE-LINC004705. E CHIP assay analysis after the cells were transfected with control, OE-NC, OE-LINC00470, and OE-LINC00470 with OE-ZNF131. F Dual-luciferase assay was used to investigate the interaction between ZNF131 and APEX1. G , H qRT-PCR and WB assay showed the expression level of APEX1 in ZNF131-overexpressed melanoma cells. qRT-PCR analysis of ZNF131 ( I ) and APEX1 ( J ) in melanoma tissues and normal tissues. K The protein expression of APEX1 and ZNF131 in melanoma and normal tissues using western blot. IHC assay was conducted to evaluate the levels of ZNF131 ( L ) and APEX1 ( M ) in melanoma and normal tissues. The mean +- SD in the graph shows the relative levels from three replicates. *p < 0.05, ** p < 0.01.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 LINC00470 promoted melanoma proliferation and metastasis through enhancing APEX1 expression in vivo. A Xenografts were shown after transfecting with shNC, shLINC00470, shLINC00470 + OE-NC, and shLINC00470 + OE-APEX1, respectively. B Tumor weight of different groups at day 28. C Tumor growth was monitored by analyzing tumor volume for 28 days. D Levels of ZNF131 and APEX1 in different groups as described in panel A were analyzed by qRT-PCR. E , F Levels of ZNF131 and APEX1 in different groups as described in panel ( A ) were analyzed by Western blot. G - I The immunohistochemical staining was conducted to determine the expression of ZNF131 ( G ), APEX1 ( H ), ki67 ( I ) in different groups as described in panel ( A ). The mean +- SD in the graph shows the relative levels from three replicates. * p < 0.05, ** p < 0.01.